Binding and activation of major histocompatibility complex class II-deficient macrophages by staphylococcal exotoxins

Macrophages from C2D transgenic mice deficient in the expression of major histocompatibility complex (MHC) class II proteins were used to identify binding sites for superantigens distinct from the MHC class II molecule. Iodinated staphylococcal enterotoxins A and B (SEA and SEB) and exfoliative toxi...

Full description

Saved in:
Bibliographic Details
Published in:Infection and Immunity 1994-09, Vol.62 (9), p.3907-3915
Main Authors: Beharka, A.A. (Kansas State University, Manhattan, KS.), Armstrong, J.W, Iandolo, J.J, Chapes, S.K
Format: Article
Language:English
Subjects:
Actins - metabolism
ANIMAL TRANSGENIQUE
ANIMALES TRANSGENICOS
Animals
ANTIGENE
ANTIGENOS
Bacteriology
Binding Sites
Biological and medical sciences
CHIMIORECEPTEUR
Cytokines - secretion
ENTEROTOXINAS
ENTEROTOXINE
Enterotoxins - metabolism
Enterotoxins - pharmacology
EXOTOXINAS
EXOTOXINE
FACTEUR IMMUNOLOGIQUE
FACTORES INMUNOLOGICOS
Fundamental and applied biological sciences. Psychology
GLOBULINAS
GLOBULINE
Histocompatibility Antigens Class II - physiology
Life Sciences (General)
MACROFAGOS
MACROPHAGE
Macrophage Activation - drug effects
Macrophages - metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microbiology
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
QUIMIORECEPTORES
RATON
SISTEMA MHC
SOURIS
Space life sciences
STAPHYLOCOCCUS AUREUS
Superantigens - metabolism
SYSTEME MHC
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Macrophages from C2D transgenic mice deficient in the expression of major histocompatibility complex (MHC) class II proteins were used to identify binding sites for superantigens distinct from the MHC class II molecule. Iodinated staphylococcal enterotoxins A and B (SEA and SEB) and exfoliative toxins A and B (ETA and ETB) bound to C2D macrophages in a concentration-dependent and competitive manner. All four toxins increased F-actin concentration within 30 s of their addition to C2D macrophages, indicating that signal transduction occurred in response to toxin in the absence of class II MHC. Furthermore, ETA, ETB, SEA, and, to a lesser extent, SEB induced C2D macrophages to produce interleukin 6. Several molecular species on C2D macrophages with molecular masses of 140, 97, 61, 52, 43, and 37 kDa bound SEA in immunoprecipitation experiments. These data indicate the presence of novel, functionally active toxin binding sites on murine macrophages distinct from MHC class II molecules
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.62.9.3907-3915.1994