Molecular phylogeny and evolution of inflorescence types in Eperua

• Published in: American journal of botany 2023-10, Vol.110 (10), p.1-e16229
• Main Authors: ter Steege, Hans, Fortes, Elenice A., Rozendaal, Danaë M. A., Erkens, Roy H. J., Sabatier, Daniel, Aymard, Gerardo, Duijm, Elza, Eurlings, Marcel, Grewe, Felix, Pombo, Maihyra M., Gomes, Vitor F., de Mansano, Vidal F., de Oliveira, Sylvia M.
• Format: Article
• Language: English
• Subjects: Bayesian analysis; Bees; Biological evolution; Chloroplasts; Eperua; Hybridization; Life Sciences; Phylogeny; Plant reproduction; Pollination; Ribosomal DNA; Statistical inference; Vegetal Biology
• ISSN: 0002-9122; 1537-2197
• DOI: 10.1002/ajb2.16229

Premise The Amazonian hyperdominant genus Eperua (Fabaceae) currently holds 20 described species and has two strongly different inflorescence and flower types, with corresponding different pollination syndrome. The evolution of these vastly different inflorescence types within this genus was unknown and the main topic in this study. Methods We constructed a molecular phylogeny, based on the full nuclear ribosomal DNA and partial plastome, using Bayesian inference and maximum likelihood methods, to test whether the genus is monophyletic, whether all species are monophyletic and if the shift from bat to bee pollination (or vice versa) occurred once in this genus. Results All but two species are well supported by the nuclear ribosomal phylogeny. The plastome phylogeny, however, shows a strong geographic signal suggesting strong local hybridization or chloroplast capture, rendering chloroplast barcodes meaningless in this genus. Conclusions With our data, we cannot fully resolve the backbone of the tree to clarify sister genera relationships and confirm monophyly of the genus Eperua. Within the genus, the shift from bat to bee and bee to bat pollination has occurred several times but, with the bee to bat not always leading to a pendant inflorescence.