Evaluation of methods for sterilising boysenberry leaves for downy mildew infection studies

Content Partner: Lincoln University. Poster presented at the New Zealand Plant Protection Conference, Nelson, 14-16 August 2012.Downy mildew of boysenberry, caused by Peronospora sparsa, is a major disease problem for New Zealand growers. Investigation of the biology and epidemiology of this biotrop...

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Main Authors: Herath Mudiyanselage, Anusara, Jaspers, Marlene, Ridgway, Hayley, Walter, M, Langford, G, Jones, Elizabeth
Format: Article
Language:English
Online Access:Request full text
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Summary:Content Partner: Lincoln University. Poster presented at the New Zealand Plant Protection Conference, Nelson, 14-16 August 2012.Downy mildew of boysenberry, caused by Peronospora sparsa, is a major disease problem for New Zealand growers. Investigation of the biology and epidemiology of this biotrophic pathogen requires the production of sporangiospore inocula. Four leaf sterilisation treatments (70% ethanol, 10% bleach, sterile water, control) were assessed for their ability to reduce surface contaminants. After treatment, the leaf surfaces (six replicates) were pressed onto potato dextrose and nutrient agar plates and these were incubated at 20°C for 1 week. All treatments significantly (P