Fish community surveys in eelgrass beds using both eDNA metabarcoding and seining: implications for biodiversity monitoring in the coastal zone

Marine Protected Areas (MPAs) have been adopted globally as a tool to combat biodiversity loss and restore marine ecosystems. Successful application of MPAs will be predicated on the ability to monitor biodiversity in a synoptic and noninvasive manner. Environmental DNA (eDNA) methods have important...

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Bibliographic Details
Published in:Canadian journal of fisheries and aquatic sciences 2022-08, Vol.79 (8), p.1335-1346
Main Authors: He, Xiaoping, Stanley, Ryan R.E., Rubidge, Emily M., Jeffery, Nicholas W., Hamilton, Lorraine C., Westfall, Kristen M., Gilmore, Scott R., Roux, Louise-Marie D., Gale, Katie S.P., Heaslip, Susan G., Steeves, Royce, Abbott, Cathryn L.
Format: Article
Language:English
Subjects:
Aquatic plants
Beaches
Biodiversity
Biodiversity loss
Coastal zone
Coasts
Conservation
Conservation areas
Distribution
DNA barcoding
Environmental aspects
Environmental DNA
Environmental monitoring
Expressed sequence tags
Fish
Fish communities
Genetic aspects
Marine ecosystems
Marine protected areas
Methods
Monitoring
Monitoring methods
Protected areas
Seining
Species richness
Surveillance
Surveys
Taxa
Water analysis
Water sampling
Wildlife conservation
Zostera marina
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Summary:Marine Protected Areas (MPAs) have been adopted globally as a tool to combat biodiversity loss and restore marine ecosystems. Successful application of MPAs will be predicated on the ability to monitor biodiversity in a synoptic and noninvasive manner. Environmental DNA (eDNA) methods have important advantages over traditional biodiversity survey methods for monitoring conservation areas. To evaluate the efficacy of eDNA metabarcoding for fish biodiversity monitoring, we sampled 19 coastal eelgrass (Zostera marina) beds in Canada, as eelgrass beds are known for high biodiversity and significant conservation value. At each site, beach seines were used to survey fish and water samples were collected contemporaneously for eDNA metabarcoding. In total, beach seining caught 32 672 individuals across 59 fish taxa, and eDNA detected 129 fish taxa. eDNA captured site-level variation and detected higher species richness at both site and regional levels compared to seining. eDNA abundance had a positive association with capture abundance. Collectively these results highlight how eDNA metabarcoding offers an efficient approach for monitoring fish biodiversity in coastal eelgrass beds, thus providing a valuable and noninvasive tool for MPA planning and coastal monitoring.
ISSN:0706-652X
1205-7533
DOI:10.1139/cjfas-2021-0215