ELECTROPHORETIC HOMOGENEITY OF POLYSACCHARIDES IN MOLAR ALKALI

The usefulness of electrophoretic patterns of polysaccharides in molar alkali as a general criterion of the purity of these substances has been studied. No clear separation of the components of a hemicellulose, of wheat starch, or of potato starch could be demonstrated. Similarly, mixtures of wheat...

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Bibliographic Details
Published in:Canadian journal of chemistry 1952-08, Vol.30 (8), p.603-607
Main Authors: Colvin, J. Ross, Cook, W. H, Adams, G. A
Format: Article
Language:English
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Summary:The usefulness of electrophoretic patterns of polysaccharides in molar alkali as a general criterion of the purity of these substances has been studied. No clear separation of the components of a hemicellulose, of wheat starch, or of potato starch could be demonstrated. Similarly, mixtures of wheat starch and inulin, pectin and sodium alginate, laminarin and sodium alginate, inulin and wheat starch plus sodium alginate could not be resolved in 1 M potassium hydroxide. Nonetheless, mixtures of laminarin and sodium alginate separated rapidly at pH 6.8 and 10.4. Contrary to previous reports, it is shown that the electrophoretic technique cannot be used with confidence as a general criterion of the purity of polysaccharides in molar alkali. However, it may be applicable for the characterization and fractionation of a limited class of polysaccharides which are soluble close to neutrality.
ISSN:0008-4042
1480-3291
DOI:10.1139/v52-073