Carbon-13 nuclear magnetic resonance studies of the cholesteryl ester - phosphatidylcholine system

Using difference spectroscopy, 13 C spin-lattice relaxation measurements on 40% egg phosphatidylcholine multilamellar liposomes (containing 25 mol% cholesteryl palmitate)-60% D 2 O indicate the ester contributes negligibly to the microviscosity of the acyl chains in the bilayers.At 37 °C, spin-spin...

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Bibliographic Details
Published in:Canadian journal of chemistry 1979-09, Vol.57 (18), p.2364-2370
Main Authors: Cushley, Robert J, Forrest, Bruce J
Format: Article
Language:English
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Summary:Using difference spectroscopy, 13 C spin-lattice relaxation measurements on 40% egg phosphatidylcholine multilamellar liposomes (containing 25 mol% cholesteryl palmitate)-60% D 2 O indicate the ester contributes negligibly to the microviscosity of the acyl chains in the bilayers.At 37 °C, spin-spin and spin-lattice relaxation measurements have been obtained for 25 mol% cholesteryl linoleate in both egg phosphatidylcholine and dipalmitoyl phosphatidylcholine multilayers and indicate: (a) T 1 values of the linoleate ester chain are 0.5 times those of the lecithin chain carbons, and (b) T 2 * = 16 ± 6 ms for all discernable linoleate carbons. At 52 °C, cholesteryl ring carbons C5 and C6 yield T 2 * values of 23 and 20 ms, respectively. These studies suggest the esters reside in an environment whose "fluidity" approaches that found in very low density lipoprotein (VLDL).
ISSN:0008-4042
1480-3291
DOI:10.1139/v79-380