Multiomics characterization of dose- and time-dependent effects of ionizing radiation on human skin keratinocytes

Ionizing radiation is used for various purposes, such as cancer treatment and medical diagnosis. As skin keratinocytes are frequently exposed to various radiation doses, the risk of developing skin diseases is also increasing. However, knowledge of the biological response of human keratinocytes to r...

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Bibliographic Details
Published in:The Korean journal of chemical engineering 2022, 39(9), 270, pp.2455-2464
Main Authors: Song, Won-Suk, Lee, Jae-Seung, Lim, Jun Woo, Kim, JiEung, Jo, Sung-Hyun, Kwon, Ji-Eun, Park, Ji-Hyeon, Choi, Sang Hyoun, Jang, Dongchan, Kim, Il Won, Jeong, Jae Hyun, Kim, Yun-Gon
Format: Article
Language:English
Subjects:
Biological effects
Biotechnology
Catalysis
Cell death
Chemistry
Chemistry and Materials Science
DNA repair
Industrial Chemistry/Chemical Engineering
Ionizing radiation
Materials Science
Metabolites
Radiation
Radiation dosage
Repair
Three dimensional models
Time dependence
화학공학
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Summary:Ionizing radiation is used for various purposes, such as cancer treatment and medical diagnosis. As skin keratinocytes are frequently exposed to various radiation doses, the risk of developing skin diseases is also increasing. However, knowledge of the biological response of human keratinocytes to radiation doses and time-points is still limited despite its importance. Here, we characterized the proteomic and metabolomic changes in HEKn cells on day 0 and 2 after irradiation with control, 0.1, 0.5, and 2 Gy to identify biological effects depending on IR dose and time. First, we found that the expression levels of proteins and metabolites involved in energy metabolism, DNA repair system, and epidermal cornification were changed following irradiation. In particular, fumarate and fumarate hydratase, which promote DNA repair, were significantly increased in response to irradiation with 2 Gy on day 2. Furthermore, all doses of IR-induced proteomic changes correlated with epidermal cornification and inhibition of cell death on day 2 after irradiation. Using a human skin 3D full-thickness model, we observed dose-dependent increases in the cornified layer and the expression of involucrin. The multiomics signatures of cellular responses depending on IR dose and time may constitute a high-value resource, providing biological effects on IR applications.
ISSN:0256-1115
1975-7220
DOI:10.1007/s11814-022-1095-2