Cytotoxicity of two self-adhesive resin cements and their interference in the phagocytic activity of murine macrophages

• Published in: Restorative dentistry & endodontics 2022, 47(3), , pp.1-9
• Main Authors: da Silva, Danilo Couto, Vaz, Leonardo Gomes, Tavares, Warley Luciano Fonseca, Vieira, Leda Quercia, de Oliveira, Ricardo Reis, Sobrinho, Antônio Paulino Ribeiro
• Format: Article
• Language: English
• Subjects: 치의학
• ISSN: 2234-7658; 2234-7666
• DOI: 10.5395/rde.2022.47.e31

ObjectivesThis study aimed to evaluate in vitro the effects of the self-adhesive resin cements RelyX U200 (3M ESPE) and seT PP (SDI Limited) on murine macrophages and the interference of the photoactivation. Materials and MethodsCell viability assays, cell adherence, yeast phagocytosis of Saccharomyces boulardii and production of reactive oxygen species (ROS) were performed in the presence of capillaries containing the respective self-adhesive cement when photoactivated or not. ResultsAfter long periods of contact, both types of cements, when not photoactivated, are more cytotoxic for macrophages. The seT PP cement when only chemically activated seems to interfere more negatively in the process of phagocytosis of yeasts S. boulardii. Both types of cements interfere in the cell adhesion process, independent of photoactivation. None of the types of cements tested was able to induce the production of ROS. ConclusionsOur results highlight the great importance of the photoactivation of self-adhesive resin cements in the dental clinic, since RelyX U200, when photoactivated, presented the best results within the evaluated parameters.