Optimal Media Conditions for the Detection of Extracellular Cellulase Activity in Ganoderma neo-japonicum

To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the use of four dyes, Congo red, phenol red, remazol brilliant blue, and trypan blue. To ob...

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Bibliographic Details
Published in:Mycobiology 2011, 39(2), , pp.129-132
Main Authors: Jo, W.S., Gyeongbuk Agricultural Technology Administration, Daegu, Republic of Korea, Park, H.N., Gyeongbuk Agricultural Technology Administration, Daegu, Republic of Korea, Cho, D.H., Gyeongbuk Agricultural Technology Administration, Daegu, Republic of Korea, Yoo, Y.B., National Institute of Horticultural and Herbal Science, RDA, Suwon, Republic of Korea, Park, S.C., Kyungpook National University, Daegu, Republic of Korea
Format: Article
Language:English
Subjects:
CELLULASE
CELULASA
Chromogenic media
Congo red
Ganoderma neo-japonicum
Research Note
생물학
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Summary:To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the use of four dyes, Congo red, phenol red, remazol brilliant blue, and trypan blue. To observe the effect of pH on the chromogenic reaction, we tested media ranging from 4.5 to 8.0. To research the effect of temperature on the clear zone and the fungus growing zone, we tested temperatures ranging from 15 to 35℃. On the whole, the best protocol called for Ganoderma neo-japonicum transfer onto media containing Congo red with a pH of 7.0, followed by incubation at 25℃ for 5 days. Our results will be useful to researchers who study extracellular enzyme activity in Ganoderma neo-japonicum.
ISSN:1229-8093
2092-9323
DOI:10.4489/MYCO.2011.39.2.129