Comparison of young male mice of two different strains (C57BL/6J and the hybrid B6129SF1/J) in selected behavior tests: a small scale study

• Published in: Laboratory animal research 2022, 38(4), , pp.287-293
• Main Authors: Hansen, Kristine Eraker Aasland, Hudecová, Alexandra M., Haugen, Fred, Skjerve, Eystein, Ropstad, Erik, Zimmer, Karin E.
• Format: Article
• Language: English
• Subjects: Barnes maze test; Behavior; Behavior test; C57BL/6J; Corticosterone; Exploratory behavior; Feces; Hybrid B6129SF1/J; Laboratory animals; Locomotor activity; Males; Medical research; Mouse strain; Open field test; Stress; Urine; 수의학
• ISSN: 2233-7660; 1738-6055; 2233-7660
• DOI: 10.1186/s42826-022-00140-5

BackgroundAll mouse strains are different, before choosing a strain for a large study, a small scale study should be done. In this study, we compared young males of two mouse strains, C57BL/6J and the hybrid B6129SF1/J, and gained knowledge on their performance in three different behavioral tests; open field (OF) test, Barnes maze (BM) test and a restraint stress test.ResultsWe found that the young males of the C57BL/6J strain spent more time moving in the OF. In the BM, the hybrid covered less ground before reaching the goal box during the first three sessions, than the C57BL/6J. The hybrid left more fecal pellets than C57BL/6J both in OF and BM. During the stress test, the C57BL/6J had a lower corticosterone response than the hybrid.ConclusionsOur findings indicate that the C57BL/6J has a presumably higher locomotor activity and/or explorative behavior than the hybrid, while the hybrid appeared more sensitive to stress.