Molecular analysis of chicken interferon-alpha inducible protein 6 gene and transcriptional regulation

• Published in: Journal of animal science and technology 2023, 65(1), , pp.183-196
• Main Authors: Park, Jeong-Woong, Ndimukaga, Marc, So, Jaerung, Kim, Sujung, Truong, Anh Duc, Tran, Ha Thi Thanh, Dang, Hoang Vu, Song, Ki-Duk
• Format: Article
• Language: English
• Subjects: Avian influenza virus; Interferon-alpha inducible protein 6 gene; MAPKs pathway, DF-1 cells; NF-κB pathway; Toll-like receptor 3 signaling pathway; 축산학
• ISSN: 2055-0391; 2672-0191; 2055-0391; 2093-6281
• DOI: 10.5187/jast.2022.e101

Interferon-alpha inducible protein 6 (IFI6) is an interferon-stimulated gene (ISG), belonging to the FAM14 family of proteins and is localized in the mitochondrial membrane, where it plays a role in apoptosis. Transcriptional regulation of this gene is poorly understood in the context of inflammation by intracellular nucleic acid-sensing receptors and pathological conditions caused by viral infection. In this study, chicken (ch ) was identified and studied for its molecular features and transcriptional regulation in chicken cells and tissues, i.e., lungs, spleens, and tracheas from highly pathogenic avian influenza virus (HPAIV)-infected chickens. The ch -coding sequences contained 1638 nucleotides encoding 107 amino acids in three exons, whereas the duck -coding sequences contained 495 nucleotides encoding 107 amino acids. IFI6 proteins from chickens, ducks, and quail contain an IF6/IF27-like superfamily domain. Expression of ch was higher in HPAIV-infected White Leghorn chicken lungs, spleens, and tracheas than in mock-infected controls. signals regulate the transcription of ch in chicken DF-1 cells via the NF-κB and JNK signaling pathways, indicating that multiple signaling pathways differentially contribute to the transcription of ch . Further research is needed to unravel the molecular mechanisms underlying transcription, as well as the involvement of ch in the pathogenesis of HPAIV in chickens.