Anti-inflammatory Activity of Dichloromethane Extract of Auricularia auricula-judae in RAW264.7 Cells

• Published in: Toxicological research (Seoul) 2011, 27(1), , pp.11-14
• Main Authors: Damte, Dereje, Kyungpook National University, Daegu, Republic of Korea, Reza, Md. Ahsanur, Kyungpook National University, Daegu, Republic of Korea, Lee, S.J., Kyungpook National University, Daegu, Republic of Korea, Jo, W.S., Gyeongbuk Agricultural Technology Administration, Daegu, Republic of Korea, Park, S.C., Kyungpook National University, Daegu, Republic of Korea
• Format: Article
• Language: English
• Subjects: Anti-inflammatory; Auricularia auricula-judae; Biomedicine; Inflammatory cytokines; NITRIC OXIDE; OXIDO NITRICO; OXYDE NITRIQUE; Pharmacology/Toxicology; 예방의학
• ISSN: 1976-8257; 2234-2753
• DOI: 10.5487/TR.2011.27.1.011

The present study investigated the anti-inflammatory effects of dichloromethane extract of Auricularia auricula-judae. Dichloromethane extract of Auricularia auricula-judae inhibited Lipopolysaccharide (LPS)-induced nitric oxide (NO) production significantly in a dose-dependent manner in the concentration greater-than or equal to 10 ㎍/ml (p less than 0.05). Furthermore, RT-PCR results of this study indicated that the extract markedly reduced the expressions of inflammatory cytokines (IL-6, TNF-α and IL-1β) mRNA in LPS-treated murine RAW 264.7 macrophages, which could possibly ameliorate the inflammation. Nevertheless, dichloromethane extract of Auricularia auricula-judae did not show complete inhibition of IL-6 mRNA expression. The inhibition of IL-1β cytokine at protein level was also observed in a dose dependent manner. In conclusion, the current study revealed the previously unknown effect of dichloromethane ethyl extract of Auricularia auricula-judae inhibitions of the production of NO, IL-6, TNF-α and IL-1β in LPS-stimulated macrophages.