Association of functional sequence variants of the myosin heavy chain 3 gene with muscle collagen content in pigs

• Published in: Journal of animal science and technology 2023, 65(3), , pp.511-518
• Main Authors: Kang, Yong-Jun, Han, Sang-Hyun, Kim, Sang-Geum, Kim, Su-Yeon, Kim, Hyeon-Ah, Kim, Yoo-Kyung, Yoo, Ji-Hyun, Shin, Moon-Cheol, Yang, Byoung-Chul, Park, Hee-Bok, Lee, Jun Heon, Cho, In-Cheol
• Format: Article
• Language: English
• Subjects: Collagen content; Genotype; Muscle; Myosin heavy chain 3; pig; 축산학
• ISSN: 2055-0391; 2672-0191; 2055-0391; 2093-6281
• DOI: 10.5187/jast.2023.e4

This study examined the association between functional sequence variants (FSVs) of ( ) genotypes and collagen content in a Landrace and Jeju native pig (JNP) crossbred population. Four muscles ( , , , and ) were used for the analysis of meat collagen content, and the same animals were genotyped for the FSVs of the gene by using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Three FSVs of genotypes were identified and had genotype frequencies of 0.358, 0.551, and 0.091 for , , and , respectively. animals for the FSVs of the genotypes showed higher collagen content in their ( < 0.001), ( < 0.001), ( < 0.001), and ( < 0.001) than homozygous animals. After the validation of this result in other independent populations, the FSVs of MYH3 genotypes can be a valuable genetic marker for improving collagen content in porcine muscles and can also be applied to increase the amount of collagen for biomedical purposes.