Molecular characterization and estimation of cellulolytic potential of gut bacteria isolated from four white grub species native to Indian Himalayas

• Published in: Journal of Asia-Pacific entomology 2023, 26(1), , pp.1-12
• Main Authors: Danu, Nutan, Paschapur, Amit, Subbanna, ARNS, Stanley, Johnson, Kumar Singh, Ashish, Bisht, Ila, Prakash Gupta, Jai
• Format: Article
• Language: English
• Subjects: 16S rRNA sequencing; Cellulolytic index; Gut bacterial diversity; Indian Himalayas; Molecular characterization; White grubs; 농학
• ISSN: 1226-8615; 1876-7990
• DOI: 10.1016/j.aspen.2022.102036

[Display omitted] •178 bacterial strains were isolated from white grubs native to Indian Himalayas.•Bacterial community was represented by two phyla: Firmicutes and Actinobacteria.•Bacterial strains were molecularly characterized through 16S rRNA sequencing.•25 cellulolytic strains were selected, Bacillus spp. showed highest cellulolytic index.•These strains are good source for agro-waste management and industrial use. An investigation was carried out to isolate, identify and molecularly characterize the cellulose-degrading bacterial isolates from the guts of four white grub species (Anomala bengalensis, Brahmina coriacea, Holotrichia longipennis and Holotrichia setticollis) native to Uttarakhand, Himalayas through 16S rRNA sequencing. A total of 178 bacterial strains were isolated from different gut compartments of selected white grub species, of which 95 bacterial isolates showed cellulose metabolizing activities in the CMC assay. Maximum degraders i.e., 38 were isolated from A. bengalensis, of which 18 were isolated from the fermentation chamber. The value of cellulolytic index ranged between 0.05 and 16 showing a variable cellulolytic activity by degraders. A total of 25 potent strains of cellulose-degrading bacteria recording cellulolytic activity > 1 were isolated and sequenced for 16S rRNA gene. Bacillus stratosphericus strain CBG4MG1 (10.78 ± 4.18), Bacillus cereus strain CBG2FC1 (10.33 ± 3.53), Bacillus sp. strain CBG3MG2 (7.28 ± 0.16) and Paenibacillus ginsengagri strain CBG1FC2 (5.66 ± 2.67) were the most potent cellulose-degrading bacteria isolated from the gut of B. coriacea, H. longipennis, H. setticollis and A. bengalensis, respectively. Thus, the cellulolytic bacteria isolated from the gut of selected white grub species may be good sources for profiling novel isolates for industrial use besides identifying eco-friendly solutions for agro-waste management.