Loading…
작약 뿌리에서 분리한 폐포 선암 세포주 A549에 대한 세포독성 화합물
작약 뿌리는 monoterpene glycoside계 물질을 함유하고 있으며, 이들 화합물은 항경련, 항염증, 항바이러스, 신경보호 및 진정효과를 나타내는 것으로 알려져 있다. 이번 연구에서는 작약 뿌리의 dichloromethane (CH 2 Cl 2 ) 및 ethyl acetate (EtOAc) 가용성 분획으로부터 세포독성 물질을 탐색하고자 하였다. 그 결과, 총 13종의 화합물을 분리할 수 있었으며, 이들의 세포독성 평가를 위해 사람 유래 폐암 선암 세포주인 A549에 처리하여 세포생존율 변화를 관찰하였다. A549에 대한 세...
Saved in:
| Published in: | Journal of applied biological chemistry 2023, 66(0), , pp.272-281 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | Korean |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | |
| container_end_page | 281 |
| container_issue | 66 |
| container_start_page | 272 |
| container_title | Journal of applied biological chemistry |
| container_volume | 66 |
| creator | 박지원 Ji Won Park 신상은 Sang-eun Shin 박해원 Haewon Park 김정아 Jeong Ah Kim 양은주 Eun-ju Yang 송경식 Kyung-sik Song |
| description | 작약 뿌리는 monoterpene glycoside계 물질을 함유하고 있으며, 이들 화합물은 항경련, 항염증, 항바이러스, 신경보호 및 진정효과를 나타내는 것으로 알려져 있다. 이번 연구에서는 작약 뿌리의 dichloromethane (CH 2 Cl 2 ) 및 ethyl acetate (EtOAc) 가용성 분획으로부터 세포독성 물질을 탐색하고자 하였다. 그 결과, 총 13종의 화합물을 분리할 수 있었으며, 이들의 세포독성 평가를 위해 사람 유래 폐암 선암 세포주인 A549에 처리하여 세포생존율 변화를 관찰하였다. A549에 대한 세포독성은 gallic acid (8) > (2S)-naringenin (9) > methyl gallate (10) > 6'-Obenzoylpaeoniflorin (7) > palmitic acid (3) 순으로 나타났다. 특히, 7은 normal cell인 MRC-5에 대한 독성은 없는 것으로 확인되었으며, 7의 A549 및 MRC-5 세포생존율에 미치는 영향에 대한 보고는 이번이 처음이다. 향후 7에 대한 세포독성 메커니즘 및 선택성과 관련된 추가연구가 필요할 것으로 판단된다.
It has been known that Paeoniae Radix (PR) contains monoterpene glycosides showing a variety of biological activities such as anti-spasmodic, anti-inflammatory, anti-viral, neuroprotective, and sedative effects. This study aimed to find the cytotoxic compounds isolated from the dichloromethane (CH 2 Cl 2 )- and ethyl acetate-soluble fractions of PR. As results, thirteen compounds (1-13) were isolated and the chemical structures were identified. In addition, the human alveolar adenocarcinoma cell line (A549) was treated with isolated compounds to determine the cytotoxic effect via evaluation of cell viability. The reduction of A549 cell viability was shown as following order; gallic acid (8) > (2S)- naringenin (9) > methyl gallate (10) > 6'-O-benzoylpaeoniflorin (7) > palmitic acid (3). Especially, 7 did not show the cytotoxicity in the human lung fibroblast cell line (MRC-5). The effect of 7 on the cell viabilities in A549 and MRC-5 is firstly reported in this study. Further study is required to find out the cytotoxic mechanism and the selectivity for the cancer cells of 7 in detail. |
| doi_str_mv | 10.3839/jabc.2023.038 |
| format | article |
| fullrecord | <record><control><sourceid>kiss_nrf_k</sourceid><recordid>TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_10270495</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><kiss_id>4032799</kiss_id><sourcerecordid>4032799</sourcerecordid><originalsourceid>FETCH-LOGICAL-k855-c551faa07102d2128e3dd27820ba8bad6b73f6f026b3d362a565e1c859f10d693</originalsourceid><addsrcrecordid>eNo9kDtLw1AYhg-iYK2OTi5ZXITUc8m5jaF4qRYK0s3hcJI0kqa2kri4OcSt0AoNIhQRBEHo4iIOFX9Qkv9gasXp4eV7eHn5ANhGsEYEkftd7bg1DDGpQSJWQAVjYplcWmgVVJDkzISWhdfBRhx3IWQIC1gB5_nTfZ5-Gdn3MHud5Q_jPJka2UdShiKdGsVoXIxmRp485-mkxGeZ8pe5YVNLlrKRDW8X2vKQje7y5N0oHidF-pbN5ptgzde9uLP1xypoHx6068dms3XUqNtNMxSUmi6lyNcacgSxh8tVHeJ5mAsMHS0c7TGHE5_5EDOHeIRhTRntIFdQ6SPoMUmqYG9Z2498FbqBGujglxcDFUbKPms3VNnNoSVpKe8u5TCIrwPV9-KeOrFPW4u3IUQ4hpxggUpv59-L1VUUXOroRlmQYC4l-QGUgYH3</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>작약 뿌리에서 분리한 폐포 선암 세포주 A549에 대한 세포독성 화합물</title><source>EZB Free E-Journals</source><creator>박지원 ; Ji Won Park ; 신상은 ; Sang-eun Shin ; 박해원 ; Haewon Park ; 김정아 ; Jeong Ah Kim ; 양은주 ; Eun-ju Yang ; 송경식 ; Kyung-sik Song</creator><creatorcontrib>박지원 ; Ji Won Park ; 신상은 ; Sang-eun Shin ; 박해원 ; Haewon Park ; 김정아 ; Jeong Ah Kim ; 양은주 ; Eun-ju Yang ; 송경식 ; Kyung-sik Song</creatorcontrib><description>작약 뿌리는 monoterpene glycoside계 물질을 함유하고 있으며, 이들 화합물은 항경련, 항염증, 항바이러스, 신경보호 및 진정효과를 나타내는 것으로 알려져 있다. 이번 연구에서는 작약 뿌리의 dichloromethane (CH 2 Cl 2 ) 및 ethyl acetate (EtOAc) 가용성 분획으로부터 세포독성 물질을 탐색하고자 하였다. 그 결과, 총 13종의 화합물을 분리할 수 있었으며, 이들의 세포독성 평가를 위해 사람 유래 폐암 선암 세포주인 A549에 처리하여 세포생존율 변화를 관찰하였다. A549에 대한 세포독성은 gallic acid (8) > (2S)-naringenin (9) > methyl gallate (10) > 6'-Obenzoylpaeoniflorin (7) > palmitic acid (3) 순으로 나타났다. 특히, 7은 normal cell인 MRC-5에 대한 독성은 없는 것으로 확인되었으며, 7의 A549 및 MRC-5 세포생존율에 미치는 영향에 대한 보고는 이번이 처음이다. 향후 7에 대한 세포독성 메커니즘 및 선택성과 관련된 추가연구가 필요할 것으로 판단된다.
It has been known that Paeoniae Radix (PR) contains monoterpene glycosides showing a variety of biological activities such as anti-spasmodic, anti-inflammatory, anti-viral, neuroprotective, and sedative effects. This study aimed to find the cytotoxic compounds isolated from the dichloromethane (CH 2 Cl 2 )- and ethyl acetate-soluble fractions of PR. As results, thirteen compounds (1-13) were isolated and the chemical structures were identified. In addition, the human alveolar adenocarcinoma cell line (A549) was treated with isolated compounds to determine the cytotoxic effect via evaluation of cell viability. The reduction of A549 cell viability was shown as following order; gallic acid (8) > (2S)- naringenin (9) > methyl gallate (10) > 6'-O-benzoylpaeoniflorin (7) > palmitic acid (3). Especially, 7 did not show the cytotoxicity in the human lung fibroblast cell line (MRC-5). The effect of 7 on the cell viabilities in A549 and MRC-5 is firstly reported in this study. Further study is required to find out the cytotoxic mechanism and the selectivity for the cancer cells of 7 in detail.</description><identifier>ISSN: 1976-0442</identifier><identifier>EISSN: 2234-7941</identifier><identifier>DOI: 10.3839/jabc.2023.038</identifier><language>kor</language><publisher>한국응용생명화학회</publisher><subject>6’-O-Benzoylpaeoniflorin ; A549 ; Cytotoxicity ; Paeoniae Radix ; 농학 ; 사람 유래 폐포 선암 세포주 ; 세포독성 ; 작약 뿌리</subject><ispartof>Journal of Applied Biological Chemistry, 2023, 66(0), , pp.272-281</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART002977392$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>박지원</creatorcontrib><creatorcontrib>Ji Won Park</creatorcontrib><creatorcontrib>신상은</creatorcontrib><creatorcontrib>Sang-eun Shin</creatorcontrib><creatorcontrib>박해원</creatorcontrib><creatorcontrib>Haewon Park</creatorcontrib><creatorcontrib>김정아</creatorcontrib><creatorcontrib>Jeong Ah Kim</creatorcontrib><creatorcontrib>양은주</creatorcontrib><creatorcontrib>Eun-ju Yang</creatorcontrib><creatorcontrib>송경식</creatorcontrib><creatorcontrib>Kyung-sik Song</creatorcontrib><title>작약 뿌리에서 분리한 폐포 선암 세포주 A549에 대한 세포독성 화합물</title><title>Journal of applied biological chemistry</title><addtitle>Journal of Applied Biological Chemistry</addtitle><description>작약 뿌리는 monoterpene glycoside계 물질을 함유하고 있으며, 이들 화합물은 항경련, 항염증, 항바이러스, 신경보호 및 진정효과를 나타내는 것으로 알려져 있다. 이번 연구에서는 작약 뿌리의 dichloromethane (CH 2 Cl 2 ) 및 ethyl acetate (EtOAc) 가용성 분획으로부터 세포독성 물질을 탐색하고자 하였다. 그 결과, 총 13종의 화합물을 분리할 수 있었으며, 이들의 세포독성 평가를 위해 사람 유래 폐암 선암 세포주인 A549에 처리하여 세포생존율 변화를 관찰하였다. A549에 대한 세포독성은 gallic acid (8) > (2S)-naringenin (9) > methyl gallate (10) > 6'-Obenzoylpaeoniflorin (7) > palmitic acid (3) 순으로 나타났다. 특히, 7은 normal cell인 MRC-5에 대한 독성은 없는 것으로 확인되었으며, 7의 A549 및 MRC-5 세포생존율에 미치는 영향에 대한 보고는 이번이 처음이다. 향후 7에 대한 세포독성 메커니즘 및 선택성과 관련된 추가연구가 필요할 것으로 판단된다.
It has been known that Paeoniae Radix (PR) contains monoterpene glycosides showing a variety of biological activities such as anti-spasmodic, anti-inflammatory, anti-viral, neuroprotective, and sedative effects. This study aimed to find the cytotoxic compounds isolated from the dichloromethane (CH 2 Cl 2 )- and ethyl acetate-soluble fractions of PR. As results, thirteen compounds (1-13) were isolated and the chemical structures were identified. In addition, the human alveolar adenocarcinoma cell line (A549) was treated with isolated compounds to determine the cytotoxic effect via evaluation of cell viability. The reduction of A549 cell viability was shown as following order; gallic acid (8) > (2S)- naringenin (9) > methyl gallate (10) > 6'-O-benzoylpaeoniflorin (7) > palmitic acid (3). Especially, 7 did not show the cytotoxicity in the human lung fibroblast cell line (MRC-5). The effect of 7 on the cell viabilities in A549 and MRC-5 is firstly reported in this study. Further study is required to find out the cytotoxic mechanism and the selectivity for the cancer cells of 7 in detail.</description><subject>6’-O-Benzoylpaeoniflorin</subject><subject>A549</subject><subject>Cytotoxicity</subject><subject>Paeoniae Radix</subject><subject>농학</subject><subject>사람 유래 폐포 선암 세포주</subject><subject>세포독성</subject><subject>작약 뿌리</subject><issn>1976-0442</issn><issn>2234-7941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNo9kDtLw1AYhg-iYK2OTi5ZXITUc8m5jaF4qRYK0s3hcJI0kqa2kri4OcSt0AoNIhQRBEHo4iIOFX9Qkv9gasXp4eV7eHn5ANhGsEYEkftd7bg1DDGpQSJWQAVjYplcWmgVVJDkzISWhdfBRhx3IWQIC1gB5_nTfZ5-Gdn3MHud5Q_jPJka2UdShiKdGsVoXIxmRp485-mkxGeZ8pe5YVNLlrKRDW8X2vKQje7y5N0oHidF-pbN5ptgzde9uLP1xypoHx6068dms3XUqNtNMxSUmi6lyNcacgSxh8tVHeJ5mAsMHS0c7TGHE5_5EDOHeIRhTRntIFdQ6SPoMUmqYG9Z2498FbqBGujglxcDFUbKPms3VNnNoSVpKe8u5TCIrwPV9-KeOrFPW4u3IUQ4hpxggUpv59-L1VUUXOroRlmQYC4l-QGUgYH3</recordid><startdate>202312</startdate><enddate>202312</enddate><creator>박지원</creator><creator>Ji Won Park</creator><creator>신상은</creator><creator>Sang-eun Shin</creator><creator>박해원</creator><creator>Haewon Park</creator><creator>김정아</creator><creator>Jeong Ah Kim</creator><creator>양은주</creator><creator>Eun-ju Yang</creator><creator>송경식</creator><creator>Kyung-sik Song</creator><general>한국응용생명화학회</general><scope>HZB</scope><scope>Q5X</scope><scope>JDI</scope><scope>ACYCR</scope></search><sort><creationdate>202312</creationdate><title>작약 뿌리에서 분리한 폐포 선암 세포주 A549에 대한 세포독성 화합물</title><author>박지원 ; Ji Won Park ; 신상은 ; Sang-eun Shin ; 박해원 ; Haewon Park ; 김정아 ; Jeong Ah Kim ; 양은주 ; Eun-ju Yang ; 송경식 ; Kyung-sik Song</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-k855-c551faa07102d2128e3dd27820ba8bad6b73f6f026b3d362a565e1c859f10d693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2023</creationdate><topic>6’-O-Benzoylpaeoniflorin</topic><topic>A549</topic><topic>Cytotoxicity</topic><topic>Paeoniae Radix</topic><topic>농학</topic><topic>사람 유래 폐포 선암 세포주</topic><topic>세포독성</topic><topic>작약 뿌리</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>박지원</creatorcontrib><creatorcontrib>Ji Won Park</creatorcontrib><creatorcontrib>신상은</creatorcontrib><creatorcontrib>Sang-eun Shin</creatorcontrib><creatorcontrib>박해원</creatorcontrib><creatorcontrib>Haewon Park</creatorcontrib><creatorcontrib>김정아</creatorcontrib><creatorcontrib>Jeong Ah Kim</creatorcontrib><creatorcontrib>양은주</creatorcontrib><creatorcontrib>Eun-ju Yang</creatorcontrib><creatorcontrib>송경식</creatorcontrib><creatorcontrib>Kyung-sik Song</creatorcontrib><collection>KISS</collection><collection>Korean Studies Information Service System (KISS) B-Type</collection><collection>KoreaScience (Open Access)</collection><collection>Korean Citation Index (Open Access)</collection><jtitle>Journal of applied biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>박지원</au><au>Ji Won Park</au><au>신상은</au><au>Sang-eun Shin</au><au>박해원</au><au>Haewon Park</au><au>김정아</au><au>Jeong Ah Kim</au><au>양은주</au><au>Eun-ju Yang</au><au>송경식</au><au>Kyung-sik Song</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>작약 뿌리에서 분리한 폐포 선암 세포주 A549에 대한 세포독성 화합물</atitle><jtitle>Journal of applied biological chemistry</jtitle><addtitle>Journal of Applied Biological Chemistry</addtitle><date>2023-12</date><risdate>2023</risdate><volume>66</volume><issue>66</issue><spage>272</spage><epage>281</epage><pages>272-281</pages><issn>1976-0442</issn><eissn>2234-7941</eissn><abstract>작약 뿌리는 monoterpene glycoside계 물질을 함유하고 있으며, 이들 화합물은 항경련, 항염증, 항바이러스, 신경보호 및 진정효과를 나타내는 것으로 알려져 있다. 이번 연구에서는 작약 뿌리의 dichloromethane (CH 2 Cl 2 ) 및 ethyl acetate (EtOAc) 가용성 분획으로부터 세포독성 물질을 탐색하고자 하였다. 그 결과, 총 13종의 화합물을 분리할 수 있었으며, 이들의 세포독성 평가를 위해 사람 유래 폐암 선암 세포주인 A549에 처리하여 세포생존율 변화를 관찰하였다. A549에 대한 세포독성은 gallic acid (8) > (2S)-naringenin (9) > methyl gallate (10) > 6'-Obenzoylpaeoniflorin (7) > palmitic acid (3) 순으로 나타났다. 특히, 7은 normal cell인 MRC-5에 대한 독성은 없는 것으로 확인되었으며, 7의 A549 및 MRC-5 세포생존율에 미치는 영향에 대한 보고는 이번이 처음이다. 향후 7에 대한 세포독성 메커니즘 및 선택성과 관련된 추가연구가 필요할 것으로 판단된다.
It has been known that Paeoniae Radix (PR) contains monoterpene glycosides showing a variety of biological activities such as anti-spasmodic, anti-inflammatory, anti-viral, neuroprotective, and sedative effects. This study aimed to find the cytotoxic compounds isolated from the dichloromethane (CH 2 Cl 2 )- and ethyl acetate-soluble fractions of PR. As results, thirteen compounds (1-13) were isolated and the chemical structures were identified. In addition, the human alveolar adenocarcinoma cell line (A549) was treated with isolated compounds to determine the cytotoxic effect via evaluation of cell viability. The reduction of A549 cell viability was shown as following order; gallic acid (8) > (2S)- naringenin (9) > methyl gallate (10) > 6'-O-benzoylpaeoniflorin (7) > palmitic acid (3). Especially, 7 did not show the cytotoxicity in the human lung fibroblast cell line (MRC-5). The effect of 7 on the cell viabilities in A549 and MRC-5 is firstly reported in this study. Further study is required to find out the cytotoxic mechanism and the selectivity for the cancer cells of 7 in detail.</abstract><pub>한국응용생명화학회</pub><doi>10.3839/jabc.2023.038</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1976-0442 |
| ispartof | Journal of Applied Biological Chemistry, 2023, 66(0), , pp.272-281 |
| issn | 1976-0442 2234-7941 |
| language | kor |
| recordid | cdi_nrf_kci_oai_kci_go_kr_ARTI_10270495 |
| source | EZB Free E-Journals |
| subjects | 6’-O-Benzoylpaeoniflorin A549 Cytotoxicity Paeoniae Radix 농학 사람 유래 폐포 선암 세포주 세포독성 작약 뿌리 |
| title | 작약 뿌리에서 분리한 폐포 선암 세포주 A549에 대한 세포독성 화합물 |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T02%3A55%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-kiss_nrf_k&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%EC%9E%91%EC%95%BD%20%EB%BF%8C%EB%A6%AC%EC%97%90%EC%84%9C%20%EB%B6%84%EB%A6%AC%ED%95%9C%20%ED%8F%90%ED%8F%AC%20%EC%84%A0%EC%95%94%20%EC%84%B8%ED%8F%AC%EC%A3%BC%20A549%EC%97%90%20%EB%8C%80%ED%95%9C%20%EC%84%B8%ED%8F%AC%EB%8F%85%EC%84%B1%20%ED%99%94%ED%95%A9%EB%AC%BC&rft.jtitle=Journal%20of%20applied%20biological%20chemistry&rft.au=%EB%B0%95%EC%A7%80%EC%9B%90&rft.date=2023-12&rft.volume=66&rft.issue=66&rft.spage=272&rft.epage=281&rft.pages=272-281&rft.issn=1976-0442&rft.eissn=2234-7941&rft_id=info:doi/10.3839/jabc.2023.038&rft_dat=%3Ckiss_nrf_k%3E4032799%3C/kiss_nrf_k%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-k855-c551faa07102d2128e3dd27820ba8bad6b73f6f026b3d362a565e1c859f10d693%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rft_kiss_id=4032799&rfr_iscdi=true |