Loading…

Morphological and macrostructural studies of dog cranial bone demineralized with different acids

Strong and weak acids were chosen for the bone decalcification process. Demineralization of dog cranial bone was carried out using 0.6 M concentration of either of hydrochloric, lactic or citric acid. Consequent morphologic changes were correlated with the developed chemistry, porosity and structure...

Full description

Saved in:
Bibliographic Details
Published in:Current applied physics 2013, 13(5), , pp.864-874
Main Authors: El-Bassyouni, Gehan T., Guirguis, Osiris W., Abdel-Fattah, Wafa I.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Strong and weak acids were chosen for the bone decalcification process. Demineralization of dog cranial bone was carried out using 0.6 M concentration of either of hydrochloric, lactic or citric acid. Consequent morphologic changes were correlated with the developed chemistry, porosity and structure through XRD and FT-IR of the matrices. The dielectric permittivity, loss angle, relaxation dielectric loss as well as a.c. electrical conductivity as functions of frequency and temperature were measured. Zeta potential was evaluated at physiologic pH and temperature and correlated with the developed structures. The results prove lower dielectric properties of demineralized matrices compared to control and exhibited high dissipation of electric energy with more than one relaxation mechanism. This variation proves that the spectral behavior depended on the nature of the bone matrix which resulted from the phase compositions of bone and its crystallite size. The behavior of the obtained results is attributed to the differences of the demineralizing acids and their consequent actions on the matrices, i.e., the differences in the materials. ►Leading markets and FDA are concerned with the development of demineralized bone. ► Microstructural studies of new routes for clinical applications in bone surgeries. ► Mechanism of demineralization in relation to type and function of acid is followed. ► Negative zeta potential of demineralized matrices to expect bone regeneration.
ISSN:1567-1739
1878-1675
DOI:10.1016/j.cap.2012.12.026