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n-Acetylcysteine protects against diazinon-induced histopathological damage and apoptosis in renal tissue of rats
Diazinon (DZN) is a member of organophosphorus insecticides that has cytotoxic effects on different organs. n -Acetyl cysteine (NAC) is a widely used antioxidant in clinical, in vivo and in vitro studies. We evaluated the protective role of NAC against DZN-induced toxicity in kidney tissue of Wistar...
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Published in: | Toxicological research (Seoul) 2024, 40(2), , pp.285-295 |
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description | Diazinon (DZN) is a member of organophosphorus insecticides that has cytotoxic effects on different organs.
n
-Acetyl cysteine (NAC) is a widely used antioxidant in clinical, in vivo and in vitro studies. We evaluated the protective role of NAC against DZN-induced toxicity in kidney tissue of Wistar rats. 30 male Wistar rats were divided into 5 groups of control, single dose of DZN, continuous dose of DZN, single doses of DZN + NAC and continuous doses of DZN + NAC. Kidney function test (blood urea nitrogen, creatinine and uric acid) was provided. Levels of malondialdehyde (MDA), total antioxidant capacity (TAC) and total sulfhydryl (T-SH) were determined in renal tissues. Renal cells apoptosis was detected using TUNEL assay. The mRNA expressions of apoptosis, oxidative stress and inflammatory mediators, including B-cell lymphoma-2 (Bcl2), Bcl-2-associated X protein (Bax), superoxide dismutase (SOD), catalase (CAT), Interleukin 10 (IL-10), Tumor necrosis factor-α (TNF-α), Caspase-3 and Caspase-8 were analyzed in kidney tissues using Real Time PCR method. Chronic exposure to DZN was associated with severe morphological changes in the kidney, as well as impairment of its function and decreased kidney weights. Continues treatment with DZN significantly decreased the percentage of renal apoptotic cells as compared to rats treated with continuous dose of DZN alone (17.69 ± 3.67% vs. 39.46% ± 2.44%;
p
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doi_str_mv | 10.1007/s43188-024-00226-3 |
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n
-Acetyl cysteine (NAC) is a widely used antioxidant in clinical, in vivo and in vitro studies. We evaluated the protective role of NAC against DZN-induced toxicity in kidney tissue of Wistar rats. 30 male Wistar rats were divided into 5 groups of control, single dose of DZN, continuous dose of DZN, single doses of DZN + NAC and continuous doses of DZN + NAC. Kidney function test (blood urea nitrogen, creatinine and uric acid) was provided. Levels of malondialdehyde (MDA), total antioxidant capacity (TAC) and total sulfhydryl (T-SH) were determined in renal tissues. Renal cells apoptosis was detected using TUNEL assay. The mRNA expressions of apoptosis, oxidative stress and inflammatory mediators, including B-cell lymphoma-2 (Bcl2), Bcl-2-associated X protein (Bax), superoxide dismutase (SOD), catalase (CAT), Interleukin 10 (IL-10), Tumor necrosis factor-α (TNF-α), Caspase-3 and Caspase-8 were analyzed in kidney tissues using Real Time PCR method. Chronic exposure to DZN was associated with severe morphological changes in the kidney, as well as impairment of its function and decreased kidney weights. Continues treatment with DZN significantly decreased the percentage of renal apoptotic cells as compared to rats treated with continuous dose of DZN alone (17.69 ± 3.67% vs. 39.46% ± 2.44%;
p
< 0.001). Continuous exposure to DZN significantly decreased TAC and T-SH contents, as well as SOD and CAT expression, but increased MDA contents in the kidney tissues (
p
< 0.001). A significant increase was observed in mRNA expression of Bax, Caspase-3, Caspase-8, as well as TNF-α following exposure to DZN, but the expression of IL-10 and Bcl2 was significantly decreased. NAC can protect kidney tissue against DZN-induced toxicity by elevating antioxidants capacity, mitigating oxidative stress, inflammation and apoptosis.</description><identifier>ISSN: 1976-8257</identifier><identifier>EISSN: 2234-2753</identifier><identifier>DOI: 10.1007/s43188-024-00226-3</identifier><identifier>PMID: 38525131</identifier><language>eng</language><publisher>Singapore: Springer Nature Singapore</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Original Article ; Pharmacology/Toxicology ; 예방의학</subject><ispartof>한국독성학회지, 2024, 40(2), , pp.285-295</ispartof><rights>The Author(s) under exclusive licence to Korean Society of Toxicology 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-87940cd3fcb1a3453d77228a15630cbdcca27058ee61db8e4f4d4226ad91d603</citedby><cites>FETCH-LOGICAL-c382t-87940cd3fcb1a3453d77228a15630cbdcca27058ee61db8e4f4d4226ad91d603</cites><orcidid>0009-0006-5951-9669</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38525131$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART003071347$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Dong, Gaiqin</creatorcontrib><creatorcontrib>Li, Qingfeng</creatorcontrib><creatorcontrib>Yu, Chun</creatorcontrib><creatorcontrib>Wang, Qing</creatorcontrib><creatorcontrib>Zuo, Danhua</creatorcontrib><creatorcontrib>Li, Xiaozhong</creatorcontrib><title>n-Acetylcysteine protects against diazinon-induced histopathological damage and apoptosis in renal tissue of rats</title><title>Toxicological research (Seoul)</title><addtitle>Toxicol Res</addtitle><addtitle>Toxicol Res</addtitle><description>Diazinon (DZN) is a member of organophosphorus insecticides that has cytotoxic effects on different organs.
n
-Acetyl cysteine (NAC) is a widely used antioxidant in clinical, in vivo and in vitro studies. We evaluated the protective role of NAC against DZN-induced toxicity in kidney tissue of Wistar rats. 30 male Wistar rats were divided into 5 groups of control, single dose of DZN, continuous dose of DZN, single doses of DZN + NAC and continuous doses of DZN + NAC. Kidney function test (blood urea nitrogen, creatinine and uric acid) was provided. Levels of malondialdehyde (MDA), total antioxidant capacity (TAC) and total sulfhydryl (T-SH) were determined in renal tissues. Renal cells apoptosis was detected using TUNEL assay. The mRNA expressions of apoptosis, oxidative stress and inflammatory mediators, including B-cell lymphoma-2 (Bcl2), Bcl-2-associated X protein (Bax), superoxide dismutase (SOD), catalase (CAT), Interleukin 10 (IL-10), Tumor necrosis factor-α (TNF-α), Caspase-3 and Caspase-8 were analyzed in kidney tissues using Real Time PCR method. Chronic exposure to DZN was associated with severe morphological changes in the kidney, as well as impairment of its function and decreased kidney weights. Continues treatment with DZN significantly decreased the percentage of renal apoptotic cells as compared to rats treated with continuous dose of DZN alone (17.69 ± 3.67% vs. 39.46% ± 2.44%;
p
< 0.001). Continuous exposure to DZN significantly decreased TAC and T-SH contents, as well as SOD and CAT expression, but increased MDA contents in the kidney tissues (
p
< 0.001). A significant increase was observed in mRNA expression of Bax, Caspase-3, Caspase-8, as well as TNF-α following exposure to DZN, but the expression of IL-10 and Bcl2 was significantly decreased. NAC can protect kidney tissue against DZN-induced toxicity by elevating antioxidants capacity, mitigating oxidative stress, inflammation and apoptosis.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Original Article</subject><subject>Pharmacology/Toxicology</subject><subject>예방의학</subject><issn>1976-8257</issn><issn>2234-2753</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNp9kU1r3DAQhkVpaZY0f6CHomMpqNWXLfm4hH4EAoWyd6GVZEeJV3I08mH766vEaY-dyxzmmRdmHoTeM_qZUaq-gBRMa0K5JJRy3hPxCu04F5Jw1YnXaMcG1RPNO3WBrgDuaatOqp4Ob9GF0B3vmGA79JjI3oV6nt0Zaogp4KXkGlwFbCcbE1Tso_0dU04kJr-64PFdhJoXW-_ynKfo7Iy9PdkpYJs8tkteaoYIOCZcQmrTGgHWgPOIi63wDr0Z7Qzh6qVfosO3r4frH-T25_eb6_0tcULzSrQaJHVejO7IrJCd8Epxri3rekHd0TtnuaKdDqFn_qiDHKWX7Q_WD8z3VFyiT1tsKqN5cNFkG5_7lM1DMftfhxvDqORSib7BHze4Hf-4BqjmFMGFebYp5BUMH3Snhk4PoqF8Q13JACWMZinxZMu5pZknM2YzY5oZ82zGPC19eMlfj6fg_6389dAAsQHQRmkKxdzntbTnwf9i_wDsYppW</recordid><startdate>20240401</startdate><enddate>20240401</enddate><creator>Dong, Gaiqin</creator><creator>Li, Qingfeng</creator><creator>Yu, Chun</creator><creator>Wang, Qing</creator><creator>Zuo, Danhua</creator><creator>Li, Xiaozhong</creator><general>Springer Nature Singapore</general><general>한국독성학회</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ACYCR</scope><orcidid>https://orcid.org/0009-0006-5951-9669</orcidid></search><sort><creationdate>20240401</creationdate><title>n-Acetylcysteine protects against diazinon-induced histopathological damage and apoptosis in renal tissue of rats</title><author>Dong, Gaiqin ; Li, Qingfeng ; Yu, Chun ; Wang, Qing ; Zuo, Danhua ; Li, Xiaozhong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-87940cd3fcb1a3453d77228a15630cbdcca27058ee61db8e4f4d4226ad91d603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Original Article</topic><topic>Pharmacology/Toxicology</topic><topic>예방의학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dong, Gaiqin</creatorcontrib><creatorcontrib>Li, Qingfeng</creatorcontrib><creatorcontrib>Yu, Chun</creatorcontrib><creatorcontrib>Wang, Qing</creatorcontrib><creatorcontrib>Zuo, Danhua</creatorcontrib><creatorcontrib>Li, Xiaozhong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Korean Citation Index</collection><jtitle>Toxicological research (Seoul)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dong, Gaiqin</au><au>Li, Qingfeng</au><au>Yu, Chun</au><au>Wang, Qing</au><au>Zuo, Danhua</au><au>Li, Xiaozhong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>n-Acetylcysteine protects against diazinon-induced histopathological damage and apoptosis in renal tissue of rats</atitle><jtitle>Toxicological research (Seoul)</jtitle><stitle>Toxicol Res</stitle><addtitle>Toxicol Res</addtitle><date>2024-04-01</date><risdate>2024</risdate><volume>40</volume><issue>2</issue><spage>285</spage><epage>295</epage><pages>285-295</pages><issn>1976-8257</issn><eissn>2234-2753</eissn><abstract>Diazinon (DZN) is a member of organophosphorus insecticides that has cytotoxic effects on different organs.
n
-Acetyl cysteine (NAC) is a widely used antioxidant in clinical, in vivo and in vitro studies. We evaluated the protective role of NAC against DZN-induced toxicity in kidney tissue of Wistar rats. 30 male Wistar rats were divided into 5 groups of control, single dose of DZN, continuous dose of DZN, single doses of DZN + NAC and continuous doses of DZN + NAC. Kidney function test (blood urea nitrogen, creatinine and uric acid) was provided. Levels of malondialdehyde (MDA), total antioxidant capacity (TAC) and total sulfhydryl (T-SH) were determined in renal tissues. Renal cells apoptosis was detected using TUNEL assay. The mRNA expressions of apoptosis, oxidative stress and inflammatory mediators, including B-cell lymphoma-2 (Bcl2), Bcl-2-associated X protein (Bax), superoxide dismutase (SOD), catalase (CAT), Interleukin 10 (IL-10), Tumor necrosis factor-α (TNF-α), Caspase-3 and Caspase-8 were analyzed in kidney tissues using Real Time PCR method. Chronic exposure to DZN was associated with severe morphological changes in the kidney, as well as impairment of its function and decreased kidney weights. Continues treatment with DZN significantly decreased the percentage of renal apoptotic cells as compared to rats treated with continuous dose of DZN alone (17.69 ± 3.67% vs. 39.46% ± 2.44%;
p
< 0.001). Continuous exposure to DZN significantly decreased TAC and T-SH contents, as well as SOD and CAT expression, but increased MDA contents in the kidney tissues (
p
< 0.001). A significant increase was observed in mRNA expression of Bax, Caspase-3, Caspase-8, as well as TNF-α following exposure to DZN, but the expression of IL-10 and Bcl2 was significantly decreased. NAC can protect kidney tissue against DZN-induced toxicity by elevating antioxidants capacity, mitigating oxidative stress, inflammation and apoptosis.</abstract><cop>Singapore</cop><pub>Springer Nature Singapore</pub><pmid>38525131</pmid><doi>10.1007/s43188-024-00226-3</doi><tpages>11</tpages><orcidid>https://orcid.org/0009-0006-5951-9669</orcidid></addata></record> |
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title | n-Acetylcysteine protects against diazinon-induced histopathological damage and apoptosis in renal tissue of rats |
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