Modulation of physiological and biochemical activities of Eugenia uniflora by green-synthesized silver nanoparticle and melatonin under drought stress

• Published in: Plant biotechnology reports 2024, 18(2), , pp.289-299
• Main Authors: Labulo, Ayomide H., David, Oyinade A., Terna, Augustine D., Omotosho, Timileyin P., Tanko, Nicholas S., Hassan, Ibrahim, Oluwole, Bosede R., Odebode, Adeyinka
• Format: Article
• Language: English
• Subjects: Accumulation; Agriculture; Biomedical and Life Sciences; Biotechnology; Cell Biology; Dehydrogenase; Dehydrogenases; Drought; Germination; Hydrogen peroxide; Leaves; Life Sciences; Melatonin; Nanoparticles; Original Article; Physiological effects; Physiology; Plant Biochemistry; Plant Sciences; Proline; Proline dehydrogenase; Seeds; Silver; Silver nitrate; Synthesis; 농학
• ISSN: 1863-5466; 1863-5474
• DOI: 10.1007/s11816-024-00887-4

The development of an effective and eco-friendly silver nanoparticle (AgNPs) to abate the effect of abiotic stress is an important area of nano-biotechnology. This study aimed to study the priming effect of plant-based green-synthesized silver nanoparticles and melatonin on the physiological and biochemical activities of drought-stressed E. uniflora . Sterilized seeds of E. uniflora were primed with 0.06 mg/l of ML-AgNPs, 0.06 mg/l of melatonin, and a nano-silver formulation of melatonin (1:1). Primed seeds were planted and subjected to 7 days under drought stress. The ML-AgNPs enhanced germination percentage, speed and vigor, and shoot elongation and induced the production of APx, CAT, and proline dehydrogenase (100% increases). Melatonin improved the activities of APx and CAT, total protein, accumulation of proline, and proline dehydrogenase (200% increases) and stabilized MDA content. Meanwhile, silver nano-formulation of melatonin increased leaves proliferation of leaves and production of APx, GPx, SOD, and CAT. Accumulation of proline and 100% upregulation of proline dehydrogenase osmo-regulated the effects of the drought, reduced MDA contents, and stabilized the excessive production of H 2 O 2 and O 2− . The ML-AgNO 3 showed an efficient delivery system of melatonin into the plant under drought stress. As a result, our research shows that melatonin in silver nano-formulation (1:1) is a useful biostimulant against drought stress.