A Light-Inducible Bidirectional Promoter Initiates Expression of Both Genes SHH2 and CFM3 in Brassica napus L

A bidirectional promoter is an intergenic region between a pair of adjacent and oppositely transcribed genes (‘head-to-head’ genes) that concurrently initiates the expression of both genes. In this work, we identified a bidirectional promoter P Bn265 with a 265 bp sequence between transcription init...

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Bibliographic Details
Published in:Journal of plant biology = Singmul Hakhoe chi 2023, 66(5), , pp.475-484
Main Authors: Zhu, Ruijia, Fu, Yu, Zhang, Li, Wei, Tao, Jiang, Xuefei, Wang, Maolin
Format: Article
Language:English
Subjects:
Bioengineering
Bioinformatics
Biomedical and Life Sciences
Brassica
Brassica napus
Chlorophyll
Chloroplasts
Codons
Conserved sequence
DNA methylation
Fluorescence microscopy
Gene expression
Genes
Genetic engineering
Genomes
Homeobox
Leaves
Life Sciences
Plant Breeding/Biotechnology
Plant Ecology
Plant Genetics and Genomics
Plant Sciences
Plant Systematics/Taxonomy/Biogeography
Proteins
Rape plants
Research Article
RNA polymerase
Splicing factors
Transcription initiation
Transgenic plants
생물학
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Summary:A bidirectional promoter is an intergenic region between a pair of adjacent and oppositely transcribed genes (‘head-to-head’ genes) that concurrently initiates the expression of both genes. In this work, we identified a bidirectional promoter P Bn265 with a 265 bp sequence between transcription initiation codons (ATG) of two genes encoding the homeodomain protein SHH2 and chloroplast GROUP II intron splicing factor CFM3, respectively, in genome of Brassica napus . The histochemical staining and fluorescence microscopy results demonstrated that P Bn265 possess bidirectional promoting activity in the various tissues of the transgenic Arabidopsis thaliana . Notably, the bidirectional expression activities of P Bn265 were significantly increased following red or blue light induction. Furthermore, the gene structure, ‘SHH2-BiP-CFM3’, appears highly conservative among various species in Brassicaceae . Considering its short sequence length, high sequence conservation and light-inducible properties, this promoter from Brassica napus most likely has application potential in bioengineering and agricultural molecular breeding.
ISSN:1226-9239
1867-0725
DOI:10.1007/s12374-022-09367-0