Anticancer activity of peptide W-0803 derived from Anoplophoa glabripennis

• Published in: Applied biological chemistry 2024, 67(0), , pp.1-7
• Main Authors: Yeom, Jae Ho, Lee, Jin-Woo, Hong, Seung Myun, Lee, Deok Jae, Park, Dong Choon, Chung, Namhyun
• Format: Article
• Language: English
• Subjects: Anoplophora glabripennis; Anti-cancer peptide; Anticancer properties; Antineoplastic drugs; Antitumor activity; Apoptosis; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Cell death; Cell migration; Cell viability; Chemistry; Chemistry and Materials Science; Human epithelial lung cancer cells; Lung cancer; Peptides; Trypsin; Trypsin treatment; Tumor cell lines; Wound healing; 농학
• ISSN: 2468-0834; 2468-0842
• DOI: 10.1186/s13765-024-00908-4

Natural compounds are known as a resource of anti-cancer agents. Anti-cancer capacity toward human epithelial lung cancer cell lines (A549, H460) was examined with and without treatment of trypsin on the extract of Anoplophora glabripennis . IC50 values without trypsin treatment were about 21.3 and 25.0 μg/mL for H460 and A549, respectively. When the extract was treated with trypsin, the IC 50 values were 16.0 and 15.6 μg/mL for H460 and A549, respectively, indicating that the trypsin treatment increased the anti-cancer capacity. Because trypsin treatment increased the capacity, the extract was treated with trypsin to isolate the peptide W-0803 which has lysin (K) in C-terminal and α-helix structures. With treatment of W-0803, the cell viability decreased dose-dependently for H460 and A549 cells. Apoptosis analysis showed that the cell death with the treatment of peptide W-0803 was mainly by apoptosis. The wound-healing assay also showed that the peptide W-0803 has an inhibitory capability on cell migration of H460 and A549 cells. All these results suggest that the peptide W-0803 is an anti-cancer agent for lung cancer cell treatment.