Saxibacter everestensis gen. nov., sp. nov., A Novel Member of the Family Brevibacteriaceae, Isolated from the North Slope of Mount Everest

• Published in: The journal of microbiology 2024, 62(4), , pp.277-284
• Main Authors: Tian, Mao, Wu, Shiyu, Zhang, Wei, Zhang, Gaosen, Yu, Xue, Wu, Yujie, Jia, Puchao, Zhang, Binglin, Chen, Tuo, Liu, Guangxiu
• Format: Article
• Language: English
• Subjects: Amino acids; Biomedical and Life Sciences; Brevibacteriaceae; Cell walls; Deoxyribonucleic acid; DNA; Evolutionary Microbiology and Systematics; Fatty acids; Gene sequencing; Genomes; Glycerol; Glycolipids; Hybridization; Life Sciences; Lipids; Microbiology; New genera; New genus; New species; Nucleotides; Nutrient requirements; Peptidoglycans; Phospholipids; Phylogenetics; Phylogeny; Quinones; Ribose; rRNA 16S; Sodium chloride; 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-024-00108-1

We isolated and analyzed a novel, Gram-stain-positive, aerobic, rod-shaped, non-motile actinobacterium, designated as strain ZFBP1038 T , from rock sampled on the north slope of Mount Everest. The growth requirements of this strain were 10–37 °C, pH 4–10, and 0–6% (w/v) NaCl. The sole respiratory quinone was MK-9, and the major fatty acids were anteiso-C 15:0 and iso-C 17:0 . Peptidoglycan containing meso-diaminopimelic acid, ribose, and glucose were the major cell wall sugars, while polar lipids included diphosphatidyl glycerol, phosphatidyl glycerol, an unidentified phospholipid, and an unidentified glycolipid. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain ZFBP1038 T has the highest similarity with Spelaeicoccus albus DSM 26341  T (96.02%). ZFBP1038 T formed a distinct monophyletic clade within the family Brevibacteriaceae and was distantly related to the genus Spelaeicoccus . The G + C content of strain ZFBP1038 T was 63.65 mol% and the genome size was 4.05 Mb. Digital DNA–DNA hybridization, average nucleotide identity, and average amino acid identity values between the genomes of strain ZFBP1038 T and representative reference strains were 19.3–25.2, 68.0–71.0, and 52.8–60.1%, respectively. Phylogenetic, phenotypic, and chemotaxonomic characteristics as well as comparative genome analyses suggested that strain ZFBP1038 T represents a novel species of a new genus, for which the name Saxibacter gen. nov., sp. nov. was assigned with the type strain Saxibacter everestensis ZFBP1038 T (= EE 014  T  = GDMCC 1.3024  T  = JCM 35335  T ).