The transcriptome response of Enterobacter sp. S-33 is modulated by low pH-stress

• Published in: Genes & genomics 2024, 46(6), , pp.671-687
• Main Authors: Kumari, Kiran, Sharma, Parva Kumar, Singh, Rajnish Prakash
• Format: Article
• Language: English
• Subjects: Acidic soils; Acidification; Adaptation; Agricultural practices; Amino acids; Animal Genetics and Genomics; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biomedical and Life Sciences; Carbohydrate metabolism; Energy metabolism; Enterobacter; Enterobacter - genetics; Enterobacter - metabolism; Gene Expression Regulation, Bacterial; Gene regulation; Human Genetics; Hydrogen-Ion Concentration; Life Sciences; Lipid metabolism; Metabolism; Microbial Genetics and Genomics; Next-generation sequencing; pH effects; Plant Genetics and Genomics; Post-translation; Research Article; Stress, Physiological - genetics; Survival; Transcriptome; Transcriptomes; Transcriptomics; Translation; 생물학
• ISSN: 1976-9571; 2092-9293; 2092-9293
• DOI: 10.1007/s13258-024-01513-x

Background Acidic environments naturally occur worldwide and uncontrolled use of agricultural practices may also cause acidification of soils. The development of acidic conditions disturbs the establishment of efficient microbial populations in their natural niches. The survival of Enterobacter species under acidic stress remains poorly understood. Objective This study aimed to investigate the survival of an environmental isolate Enterobacter sp. S-33 under acidic stress and to identify the various genes involved in stress protection at the global gene transcription level. The obtained results provide new targets that will allow understanding the in-depth mechanisms involved in the adaptation of bacteria to environmental pH changes. Methods We used the next-generation sequencing (NGS) method to analyze the expression (up-regulation & down-regulation) of genes under varying pH conditions. Results A total of 4214 genes were differentially expressed under acidic conditions (pH 5.0), with 294 up-regulated and 167 down-regulated. At pH 6.0, 50 genes were significantly expressed, of which 34 and 16 were identified as up-regulated and down-regulated, respectively. Many of the up-regulated genes were involved in carbohydrate metabolism, amino acid transport & metabolism, and the most down-regulated genes were related to post-translational modification, lipid transport & metabolism, etc. The observed transcriptomic regulation of genes and pathways identified that Enterobacter reduced its post-translational modification, lipid transport & metabolism, and increased carbohydrate metabolism, amino acid metabolism & transport, energy production & conversion to adapt and grow in acidic stress. Conclusions The present work provides in-depth information on the characterization of genes associated with tolerance or adaptation to acidic stress of Enterobacter bacterium.