Effect of proton-beam irradiation on cell survival of MCF-7 and its chemo-resistant subgroups

Proton beam (PB) therapy is gaining popularity for breast cancer treatment because of its enhanced coverage and potential to minimize late toxicities. The dose and volume of PB irradiation are closely associated with improved breast cancer survival. We aimed to investigate the impact of PB irradiati...

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Bibliographic Details
Published in:Journal of the Korean Physical Society 2024, 85(3), , pp.271-278
Main Authors: Maharjan, Sushma, Lee, Min-Gu, Nam, Kyung-Soo, Lee, Kyu-Shik
Format: Article
Language:English
Subjects:
Breast cancer
DNA repair
Doxorubicin
Effectiveness
Impact resistance
Mathematical and Computational Physics
Original Paper - Cross-Disciplinary Physics and Related Areas of Science and Technology
Particle and Nuclear Physics
Physics
Physics and Astronomy
Proton beams
Radiation dosage
Subgroups
Survival
Theoretical
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Summary:Proton beam (PB) therapy is gaining popularity for breast cancer treatment because of its enhanced coverage and potential to minimize late toxicities. The dose and volume of PB irradiation are closely associated with improved breast cancer survival. We aimed to investigate the impact of PB irradiation on the survival of the human breast cancer cells MCF-7 and their resistance to doxorubicin (MCF-7-DR) and paclitaxel (MCF-7-PR). Cells exposed to 0.5, 2, 4, or 8 Gy of PB irradiation showed a significant decrease in the survival of parent cells, even at 2 Gy, indicating therapeutic efficacy. Conversely, drug-resistant cells exhibited notable cytotoxicity at 4 and 8 Gy, which were above the daily recommended dose. Mechanistically, PB irradiation significantly altered the DNA repair proteins RAD51, Ku80, and survivin and cleaved PARP in MCF-7 cells compared to chemo-resistant cells, except for RAD51 and Ku80. In addition, cell-cycle regulators and MAPK expression were notably altered by PB irradiation compared to MCF-7-DR and PR cells, underscoring the importance of tailoring PB irradiation for enhanced efficacy against chemo-resistant breast cancer. These findings suggest that PB irradiation downregulated RAD51 and Ku80, the potent DNA repair markers, underscoring its potential therapeutic efficacy in treating chemo-resistant breast cancer cells.
ISSN:0374-4884
1976-8524
DOI:10.1007/s40042-024-01089-5