Influence of aqueous extract of Annona muricata leaves in Tris-egg yolk extender on storage of spermatozoa from West African Dwarf goat ( Capra hircus )

Background: Because oxidative stress can induce decreased quality of caprine semen during the storage, there has been limitation for the use of stored semen in the assisted reproductive technologies. The present study, therefore, assesses the potential of Annona muricata (A. muricata ) to reduce sem...

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Published in:Journal of animal reproduction & biotechnology (Online) 2024, 39(3), , pp.179-193
Main Authors: Adamou, Mohamadou, Baulland, Dongmo Nguedia Arius, Pierrette, Ngo Bahebeck, Herve, Tchoffo, Momo, Chongsi Margaret Mary, Claude, Noudjio Kezeter, Mvondo, Nnanga Germaine Estelle, Nathalie, Ngwemetah, Njeiri, Adamu Yusufa, Ferdinand, Ngoula
Format: Article
Language:English
Subjects:
annona muricata
buck semen
liquid semen storage
storage-associated damages
tris-egg yolk extender
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Summary:Background: Because oxidative stress can induce decreased quality of caprine semen during the storage, there has been limitation for the use of stored semen in the assisted reproductive technologies. The present study, therefore, assesses the potential of Annona muricata (A. muricata ) to reduce semen storage associateddamages. Methods: Semen was collected by electro-ejaculation from ten bucks, and extended with Tris-egg yolk (TEY) supplemented with A. muricata leaf aqueous extract (SAE) at 20 (SAE20), 40 (SAE40), and 80 (SAE80) μg/mL. Sperm variables including motility, motion characteristics, viability, membrane functionality, and DNA integrity were assessed at different storage periods (6, 24, 48, and 72 hr). In addition, oxidative stress indicators in the extender supplemted with SAE were also assessed for each group. Results: By adding SAE at 80 μg/mL in TEY, the storage of goat buck semen was improved, resulting in reduced loss of sperm motility, viability, DNA fragmentation, and membrane integrity during chilled storage at 4℃ for up to 72 hr. In addition, enrichment of TEY extender with SAE significantly (p < 0.05) reduced malondialdehyde, an indicator of oxidative stress, compared to the negative control. Conclusions: Supplementation of SAE in TEY extender can reduce buck spermatozoa liquid storage associated damages due to oxidative stress.
ISSN:2671-4639
2671-4663
DOI:10.12750/JARB.39.3.179