Loading…

Different development patterns of reward behaviors induced by ketamine and JWH-018 in striatal GAD67 knockdown mice

Glutamic acid decarboxylase 67 (GAD67) is a gamma-aminobutyric acid (GABA) synthesis enzyme associated with the function of other neurotransmitter receptors, such as the N-methyl-D-aspartate (NMDA) receptor and cannabinoid receptor 1. However, the role of GAD67 in the development of different abused...

Full description

Saved in:
Bibliographic Details
Published in:Journal of veterinary science (Suwŏn-si, Korea) 2024, 25(5), , pp.0-0
Main Authors: Gu, Sun Mi, Hong, Eunchong, Seo, Sowoon, Kim, Sanghyeon, Yoon, Seong Shoon, Cha, Hye Jin, Yun, Jaesuk
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c301t-9271f07ba0451224e31220e4df2c4f9169861e3e38091f23ed37da979d5538263
container_end_page 0
container_issue 5
container_start_page e63
container_title Journal of veterinary science (Suwŏn-si, Korea)
container_volume 25
creator Gu, Sun Mi
Hong, Eunchong
Seo, Sowoon
Kim, Sanghyeon
Yoon, Seong Shoon
Cha, Hye Jin
Yun, Jaesuk
description Glutamic acid decarboxylase 67 (GAD67) is a gamma-aminobutyric acid (GABA) synthesis enzyme associated with the function of other neurotransmitter receptors, such as the N-methyl-D-aspartate (NMDA) receptor and cannabinoid receptor 1. However, the role of GAD67 in the development of different abused drug-induced reward behaviors remains unknown. In order to elucidate the mechanisms of substance use disorder, it is crucial to study changes in biomarkers within the brain's reward circuit induced by drug use. The study was designed to examine the effects of the downregulation of GAD67 expression in the dorsal striatum on reward behavior development. We evaluated the effects of GAD67 knockdown on depression-like behavior and anxiety using the forced swim test and elevated plus maze test in a mouse model. We further determined the effects of GAD67 knockdown on ketamine- and JWH-018-induced conditioned place preference (CPP). Knockdown of GAD67 in the dorsal striatum of mice increased depression-like behavior, but it decreased anxiety. Moreover, the CPP score on the NMDA receptor antagonist ketamine was increased by GAD67 knockdown, whereas the administration of JWH-018, a cannabinoid receptor agonist, did not affect the CPP score in the GAD67 knockdown mice group compared with the control group. These results suggest that striatal GAD67 reduces GABAergic neuronal activity and may cause ketamine-induced NMDA receptor inhibition. Consequently, GAD67 downregulation induces vulnerability to the drug reward behavior of ketamine.
doi_str_mv 10.4142/jvs.23325
format article
fullrecord <record><control><sourceid>proquest_nrf_k</sourceid><recordid>TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_10624604</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3100914451</sourcerecordid><originalsourceid>FETCH-LOGICAL-c301t-9271f07ba0451224e31220e4df2c4f9169861e3e38091f23ed37da979d5538263</originalsourceid><addsrcrecordid>eNpVkU1v1DAQhi0EoqVw4A8gHwEpxZ9JfEKrln6gSkioiN4srz2mbhJ7a2e36r-vu1squHhGnsfvzPhF6D0lh4IK9uVmUw4Z50y-QPtUdW0jpbx6WXPGVNMLebWH3pRyQ0hFWvUa7XHFOO36fh-V4-A9ZIgzdrCBMa2mx3xl5hlyLDh5nOHOZIeXcG02IeWCQ3RrC_XmHg8wmylEwCY6_P33WUNoX-u4zDmY2Yz4dHHcdniIyQ4u3UU8BQtv0StvxgLvnuIB-nXy7fLorLn4cXp-tLhoLCd0bhTrqCfd0hAh6yYCeD0JCOeZFV7RVvUtBQ68J4p6xsHxzhnVKScl71nLD9DnnW7MXg826GTCNv5Jesh68fPyXFPSMtESUeGvO3i1Xk7gbP2FbEa9ymEy-X779P9KDNdVaKMpFZJwxavCxyeFnG7XUGY9hWJhHE2EtC6aU1IHFXWZin7aoTanUjL45z6U6EdLdbVUby2t7Id_B3sm_3rIHwB7x5u0</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3100914451</pqid></control><display><type>article</type><title>Different development patterns of reward behaviors induced by ketamine and JWH-018 in striatal GAD67 knockdown mice</title><source>PubMed Central</source><creator>Gu, Sun Mi ; Hong, Eunchong ; Seo, Sowoon ; Kim, Sanghyeon ; Yoon, Seong Shoon ; Cha, Hye Jin ; Yun, Jaesuk</creator><creatorcontrib>Gu, Sun Mi ; Hong, Eunchong ; Seo, Sowoon ; Kim, Sanghyeon ; Yoon, Seong Shoon ; Cha, Hye Jin ; Yun, Jaesuk</creatorcontrib><description>Glutamic acid decarboxylase 67 (GAD67) is a gamma-aminobutyric acid (GABA) synthesis enzyme associated with the function of other neurotransmitter receptors, such as the N-methyl-D-aspartate (NMDA) receptor and cannabinoid receptor 1. However, the role of GAD67 in the development of different abused drug-induced reward behaviors remains unknown. In order to elucidate the mechanisms of substance use disorder, it is crucial to study changes in biomarkers within the brain's reward circuit induced by drug use. The study was designed to examine the effects of the downregulation of GAD67 expression in the dorsal striatum on reward behavior development. We evaluated the effects of GAD67 knockdown on depression-like behavior and anxiety using the forced swim test and elevated plus maze test in a mouse model. We further determined the effects of GAD67 knockdown on ketamine- and JWH-018-induced conditioned place preference (CPP). Knockdown of GAD67 in the dorsal striatum of mice increased depression-like behavior, but it decreased anxiety. Moreover, the CPP score on the NMDA receptor antagonist ketamine was increased by GAD67 knockdown, whereas the administration of JWH-018, a cannabinoid receptor agonist, did not affect the CPP score in the GAD67 knockdown mice group compared with the control group. These results suggest that striatal GAD67 reduces GABAergic neuronal activity and may cause ketamine-induced NMDA receptor inhibition. Consequently, GAD67 downregulation induces vulnerability to the drug reward behavior of ketamine.</description><identifier>ISSN: 1229-845X</identifier><identifier>ISSN: 1976-555X</identifier><identifier>EISSN: 1976-555X</identifier><identifier>DOI: 10.4142/jvs.23325</identifier><identifier>PMID: 39231788</identifier><language>eng</language><publisher>Korea (South): The Korean Society of Veterinary Science</publisher><subject>Animals ; Anxiety ; Corpus Striatum - drug effects ; Corpus Striatum - metabolism ; Depression - chemically induced ; Gene Knockdown Techniques ; Glutamate Decarboxylase - genetics ; Glutamate Decarboxylase - metabolism ; Indoles - pharmacology ; Ketamine - pharmacology ; Male ; Mice ; Mice, Inbred C57BL ; Naphthalenes - pharmacology ; Research Report ; Reward ; 수의학</subject><ispartof>Journal of Veterinary Science, 2024, 25(5), , pp.0-0</ispartof><rights>2024 The Korean Society of Veterinary Science.</rights><rights>2024 The Korean Society of Veterinary Science 2024 The Korean Society of Veterinary Science</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c301t-9271f07ba0451224e31220e4df2c4f9169861e3e38091f23ed37da979d5538263</cites><orcidid>0000-0002-3093-8919 ; 0000-0002-7907-0487 ; 0009-0007-4882-6492 ; 0009-0006-8239-9570 ; 0009-0003-8823-3035 ; 0000-0002-8182-1024 ; 0009-0002-9523-0759</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450393/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450393/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39231788$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART003123871$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Sun Mi</creatorcontrib><creatorcontrib>Hong, Eunchong</creatorcontrib><creatorcontrib>Seo, Sowoon</creatorcontrib><creatorcontrib>Kim, Sanghyeon</creatorcontrib><creatorcontrib>Yoon, Seong Shoon</creatorcontrib><creatorcontrib>Cha, Hye Jin</creatorcontrib><creatorcontrib>Yun, Jaesuk</creatorcontrib><title>Different development patterns of reward behaviors induced by ketamine and JWH-018 in striatal GAD67 knockdown mice</title><title>Journal of veterinary science (Suwŏn-si, Korea)</title><addtitle>J Vet Sci</addtitle><description>Glutamic acid decarboxylase 67 (GAD67) is a gamma-aminobutyric acid (GABA) synthesis enzyme associated with the function of other neurotransmitter receptors, such as the N-methyl-D-aspartate (NMDA) receptor and cannabinoid receptor 1. However, the role of GAD67 in the development of different abused drug-induced reward behaviors remains unknown. In order to elucidate the mechanisms of substance use disorder, it is crucial to study changes in biomarkers within the brain's reward circuit induced by drug use. The study was designed to examine the effects of the downregulation of GAD67 expression in the dorsal striatum on reward behavior development. We evaluated the effects of GAD67 knockdown on depression-like behavior and anxiety using the forced swim test and elevated plus maze test in a mouse model. We further determined the effects of GAD67 knockdown on ketamine- and JWH-018-induced conditioned place preference (CPP). Knockdown of GAD67 in the dorsal striatum of mice increased depression-like behavior, but it decreased anxiety. Moreover, the CPP score on the NMDA receptor antagonist ketamine was increased by GAD67 knockdown, whereas the administration of JWH-018, a cannabinoid receptor agonist, did not affect the CPP score in the GAD67 knockdown mice group compared with the control group. These results suggest that striatal GAD67 reduces GABAergic neuronal activity and may cause ketamine-induced NMDA receptor inhibition. Consequently, GAD67 downregulation induces vulnerability to the drug reward behavior of ketamine.</description><subject>Animals</subject><subject>Anxiety</subject><subject>Corpus Striatum - drug effects</subject><subject>Corpus Striatum - metabolism</subject><subject>Depression - chemically induced</subject><subject>Gene Knockdown Techniques</subject><subject>Glutamate Decarboxylase - genetics</subject><subject>Glutamate Decarboxylase - metabolism</subject><subject>Indoles - pharmacology</subject><subject>Ketamine - pharmacology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Naphthalenes - pharmacology</subject><subject>Research Report</subject><subject>Reward</subject><subject>수의학</subject><issn>1229-845X</issn><issn>1976-555X</issn><issn>1976-555X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpVkU1v1DAQhi0EoqVw4A8gHwEpxZ9JfEKrln6gSkioiN4srz2mbhJ7a2e36r-vu1squHhGnsfvzPhF6D0lh4IK9uVmUw4Z50y-QPtUdW0jpbx6WXPGVNMLebWH3pRyQ0hFWvUa7XHFOO36fh-V4-A9ZIgzdrCBMa2mx3xl5hlyLDh5nOHOZIeXcG02IeWCQ3RrC_XmHg8wmylEwCY6_P33WUNoX-u4zDmY2Yz4dHHcdniIyQ4u3UU8BQtv0StvxgLvnuIB-nXy7fLorLn4cXp-tLhoLCd0bhTrqCfd0hAh6yYCeD0JCOeZFV7RVvUtBQ68J4p6xsHxzhnVKScl71nLD9DnnW7MXg826GTCNv5Jesh68fPyXFPSMtESUeGvO3i1Xk7gbP2FbEa9ymEy-X779P9KDNdVaKMpFZJwxavCxyeFnG7XUGY9hWJhHE2EtC6aU1IHFXWZin7aoTanUjL45z6U6EdLdbVUby2t7Id_B3sm_3rIHwB7x5u0</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>Gu, Sun Mi</creator><creator>Hong, Eunchong</creator><creator>Seo, Sowoon</creator><creator>Kim, Sanghyeon</creator><creator>Yoon, Seong Shoon</creator><creator>Cha, Hye Jin</creator><creator>Yun, Jaesuk</creator><general>The Korean Society of Veterinary Science</general><general>대한수의학회</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>ACYCR</scope><orcidid>https://orcid.org/0000-0002-3093-8919</orcidid><orcidid>https://orcid.org/0000-0002-7907-0487</orcidid><orcidid>https://orcid.org/0009-0007-4882-6492</orcidid><orcidid>https://orcid.org/0009-0006-8239-9570</orcidid><orcidid>https://orcid.org/0009-0003-8823-3035</orcidid><orcidid>https://orcid.org/0000-0002-8182-1024</orcidid><orcidid>https://orcid.org/0009-0002-9523-0759</orcidid></search><sort><creationdate>20240901</creationdate><title>Different development patterns of reward behaviors induced by ketamine and JWH-018 in striatal GAD67 knockdown mice</title><author>Gu, Sun Mi ; Hong, Eunchong ; Seo, Sowoon ; Kim, Sanghyeon ; Yoon, Seong Shoon ; Cha, Hye Jin ; Yun, Jaesuk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-9271f07ba0451224e31220e4df2c4f9169861e3e38091f23ed37da979d5538263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Animals</topic><topic>Anxiety</topic><topic>Corpus Striatum - drug effects</topic><topic>Corpus Striatum - metabolism</topic><topic>Depression - chemically induced</topic><topic>Gene Knockdown Techniques</topic><topic>Glutamate Decarboxylase - genetics</topic><topic>Glutamate Decarboxylase - metabolism</topic><topic>Indoles - pharmacology</topic><topic>Ketamine - pharmacology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Naphthalenes - pharmacology</topic><topic>Research Report</topic><topic>Reward</topic><topic>수의학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Sun Mi</creatorcontrib><creatorcontrib>Hong, Eunchong</creatorcontrib><creatorcontrib>Seo, Sowoon</creatorcontrib><creatorcontrib>Kim, Sanghyeon</creatorcontrib><creatorcontrib>Yoon, Seong Shoon</creatorcontrib><creatorcontrib>Cha, Hye Jin</creatorcontrib><creatorcontrib>Yun, Jaesuk</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Korean Citation Index</collection><jtitle>Journal of veterinary science (Suwŏn-si, Korea)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Sun Mi</au><au>Hong, Eunchong</au><au>Seo, Sowoon</au><au>Kim, Sanghyeon</au><au>Yoon, Seong Shoon</au><au>Cha, Hye Jin</au><au>Yun, Jaesuk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Different development patterns of reward behaviors induced by ketamine and JWH-018 in striatal GAD67 knockdown mice</atitle><jtitle>Journal of veterinary science (Suwŏn-si, Korea)</jtitle><addtitle>J Vet Sci</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>25</volume><issue>5</issue><spage>e63</spage><epage>0</epage><pages>e63-0</pages><issn>1229-845X</issn><issn>1976-555X</issn><eissn>1976-555X</eissn><abstract>Glutamic acid decarboxylase 67 (GAD67) is a gamma-aminobutyric acid (GABA) synthesis enzyme associated with the function of other neurotransmitter receptors, such as the N-methyl-D-aspartate (NMDA) receptor and cannabinoid receptor 1. However, the role of GAD67 in the development of different abused drug-induced reward behaviors remains unknown. In order to elucidate the mechanisms of substance use disorder, it is crucial to study changes in biomarkers within the brain's reward circuit induced by drug use. The study was designed to examine the effects of the downregulation of GAD67 expression in the dorsal striatum on reward behavior development. We evaluated the effects of GAD67 knockdown on depression-like behavior and anxiety using the forced swim test and elevated plus maze test in a mouse model. We further determined the effects of GAD67 knockdown on ketamine- and JWH-018-induced conditioned place preference (CPP). Knockdown of GAD67 in the dorsal striatum of mice increased depression-like behavior, but it decreased anxiety. Moreover, the CPP score on the NMDA receptor antagonist ketamine was increased by GAD67 knockdown, whereas the administration of JWH-018, a cannabinoid receptor agonist, did not affect the CPP score in the GAD67 knockdown mice group compared with the control group. These results suggest that striatal GAD67 reduces GABAergic neuronal activity and may cause ketamine-induced NMDA receptor inhibition. Consequently, GAD67 downregulation induces vulnerability to the drug reward behavior of ketamine.</abstract><cop>Korea (South)</cop><pub>The Korean Society of Veterinary Science</pub><pmid>39231788</pmid><doi>10.4142/jvs.23325</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-3093-8919</orcidid><orcidid>https://orcid.org/0000-0002-7907-0487</orcidid><orcidid>https://orcid.org/0009-0007-4882-6492</orcidid><orcidid>https://orcid.org/0009-0006-8239-9570</orcidid><orcidid>https://orcid.org/0009-0003-8823-3035</orcidid><orcidid>https://orcid.org/0000-0002-8182-1024</orcidid><orcidid>https://orcid.org/0009-0002-9523-0759</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1229-845X
ispartof Journal of Veterinary Science, 2024, 25(5), , pp.0-0
issn 1229-845X
1976-555X
1976-555X
language eng
recordid cdi_nrf_kci_oai_kci_go_kr_ARTI_10624604
source PubMed Central
subjects Animals
Anxiety
Corpus Striatum - drug effects
Corpus Striatum - metabolism
Depression - chemically induced
Gene Knockdown Techniques
Glutamate Decarboxylase - genetics
Glutamate Decarboxylase - metabolism
Indoles - pharmacology
Ketamine - pharmacology
Male
Mice
Mice, Inbred C57BL
Naphthalenes - pharmacology
Research Report
Reward
수의학
title Different development patterns of reward behaviors induced by ketamine and JWH-018 in striatal GAD67 knockdown mice
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T22%3A25%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_nrf_k&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Different%20development%20patterns%20of%20reward%20behaviors%20induced%20by%20ketamine%20and%20JWH-018%20in%20striatal%20GAD67%20knockdown%20mice&rft.jtitle=Journal%20of%20veterinary%20science%20(Suw%C5%8Fn-si,%20Korea)&rft.au=Gu,%20Sun%20Mi&rft.date=2024-09-01&rft.volume=25&rft.issue=5&rft.spage=e63&rft.epage=0&rft.pages=e63-0&rft.issn=1229-845X&rft.eissn=1976-555X&rft_id=info:doi/10.4142/jvs.23325&rft_dat=%3Cproquest_nrf_k%3E3100914451%3C/proquest_nrf_k%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c301t-9271f07ba0451224e31220e4df2c4f9169861e3e38091f23ed37da979d5538263%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3100914451&rft_id=info:pmid/39231788&rfr_iscdi=true