Cell Death Inhibition Effect of Antioxidant Activity by 630 and 850 nm LEDs in RAW264.7 Cells

• Published in: Current optics and photonics 2024, 8(5), , pp.441-455
• Main Authors: Kim, Hee Eun, Kim, Eun Young, Ahn, Jin Chul, Mo, Sang Joon
• Format: Article
• Language: English
• Subjects: Cell death; Flow cytometry; Hydrogen peroxide; Irradiation; Light emitting diodes; Scavenging; Semiconductor lasers; 물리학
• ISSN: 2508-7266; 2508-7274
• DOI: 10.3807/COPP.2024.8.5.441

This study objective was to evaluate the effects and mechanisms of low-level laser therapy in H2O2-induced cell death in mouse macrophage RAW264.7 cell. After irradiation with 630 and 850 nm wavelength diode lasers with an intensity of 10 mW/cm2 in RAW264.7 cells treated with 0.7 Mm H2O2, the effects and mechanisms of the two wavelengths on cell death inhibition were evaluated using MTT assay, ROS staining, TUNEL assay, flow cytometry analysis, and Western blot analysis. As a result, 630 or 850 nm light-emitting diodes (LED) were irradiated for 10 or 40 minutes to increase cell viability with H2O2 by about 1.7- or 1.6-fold, respectively. In addition, irradiation with two LEDs showed significant ROS scavenging effects, and TUNEL-positive cells were significantly reduced by 45.7% (630 nm) and 37.8% (850 nm) compared to cells treated with H2O2 alone. The Bax/Bcl-2 ratio of cells irradiated with both LEDs was significantly lower than that of cells treated with H2O2 only, and the expression of procaspase-3 and cleaved PARP was also significantly expressed in the direction of suppressing cell death. In conclusion, ROS scavenging activity by both LEDs irradiation leads to the expression of cell death pathway proteins in the direction of inhibiting cell death.