In Vitro Antioxidant Activity and Effect of Parkia biglobosa Bark Extract on Mitochondrial Redox Status

Aqueous-methanolic extract of Parkia biglobosa bark (PBB) was screened for its polyphenolic constituents, in vitro antioxidant activity, and effect on mitochondria redox status. The in vitro antioxidant activity was assessed by using the scavenging abilities and the reducing powers of 1,1-diphenyl-2...

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Published in:Journal of acupuncture and meridian studies 2014, 7(4), 30, pp.202-210
Main Authors: Komolafe, Kayode, Olaleye, Tolulope Mary, Omotuyi, Olaposi Idowu, Boligon, Aline Augusti, Athayde, Margareth Linde, Akindahunsi, Akintunde Afolabi, Rocha, Joao Batista Teixeira da
Format: Article
Language:English
Subjects:
Animals
antioxidant
Antioxidants - pharmacology
Benzothiazoles - metabolism
Biphenyl Compounds - metabolism
Brain - drug effects
extract
Fabaceae - chemistry
HPLC
Lipid Peroxidation - drug effects
Liver - drug effects
Male
Membrane Potential, Mitochondrial - drug effects
mitochondria
Mitochondria - drug effects
Oxidation-Reduction
Parkia biglobosa
phenolics
Picrates - metabolism
Plant Bark
Plant Extracts - pharmacology
Polyphenols - pharmacology
Rats, Wistar
Sulfonic Acids - metabolism
한의학
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Summary:Aqueous-methanolic extract of Parkia biglobosa bark (PBB) was screened for its polyphenolic constituents, in vitro antioxidant activity, and effect on mitochondria redox status. The in vitro antioxidant activity was assessed by using the scavenging abilities and the reducing powers of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation against Fe3+. Subsequently, the ability of PBB to inhibit lipid peroxidation induced by FeSO4 (10 μm) and its metal-chelating potential were investigated. The effects of the extract on basal reactive oxygen species (ROS) generation and on the mitochondrial membrane potential (ΔΨm) in isolated mitochondria were determined by using 2′, 7′-dichlorodihydrofluorescin (DCFH) oxidation and safranin fluorescence, respectively. PBB mitigated the Fe(II)-induced lipid peroxidation in rat tissues and showed dose-dependent scavenging of DPPH (IC50: 98.33 ± 10.0 μg/mL) and ABTS. (trolox equivalent antioxidant concentration, TEAC value = 0.05), with considerable ferric-reducing and moderate metal-chelating abilities. PBB caused slight decreases in both the liver and the brain mitochondria potentials and resulted in a significant decrease (p 
ISSN:2005-2901
2093-8152
DOI:10.1016/j.jams.2013.08.003