Loading…

Induction of Apoptosis by Ethanol Extracts of Ganoderma lucidum in Human Gastric Carcinoma Cells

Ganoderma lucidum, a well-known medicinal mushroom, is highly valued and commonly used in Oriental medicine. Although recent experimental data has revealed the proapoptotic potency of G. lucidum extracts, the underlying mechanisms of this apoptotic activity have not yet been studied in detail. In th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of acupuncture and meridian studies 2010, 3(1), 7, pp.24-31
Main Authors: Jang, Kyung-Jun, Han, Min-Ho, Lee, Byung-Hoon, Kim, Byung-Woo, Kim, Cheol-Hong, Yoon, Hyun-Min, Choi, Yung-Hyun
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c511t-566c4f3923efb66fe8c0daecb635f7807272520b8bc51cdc87d29cf0f5c6bad23
cites cdi_FETCH-LOGICAL-c511t-566c4f3923efb66fe8c0daecb635f7807272520b8bc51cdc87d29cf0f5c6bad23
container_end_page 31
container_issue 1
container_start_page 24
container_title Journal of acupuncture and meridian studies
container_volume 3
creator Jang, Kyung-Jun
Han, Min-Ho
Lee, Byung-Hoon
Kim, Byung-Woo
Kim, Cheol-Hong
Yoon, Hyun-Min
Choi, Yung-Hyun
description Ganoderma lucidum, a well-known medicinal mushroom, is highly valued and commonly used in Oriental medicine. Although recent experimental data has revealed the proapoptotic potency of G. lucidum extracts, the underlying mechanisms of this apoptotic activity have not yet been studied in detail. In the present study, the effects of ethanol extracts of G. lucidum (EGL) on the growth of an AGS human gastric carcinoma cell line were investigated. We found that EGL treatment resulted in a dose and time-dependent significant decrease in the viability of AGS cells. This decreased viability was caused by apoptotic cell death, with observed chromatin condensation and an accumulation of apoptotic fraction. EGL treatment induced the expression of death receptor-related proteins such as death receptor 5 and tumor necrosis factor-related apoptosis-inducing ligand, which further triggered the activation of caspase-8 and the cleavage of Bid. In addition, the increase in apoptosis that was induced by EGL was correlated with activation of caspase-9 and -3, downregulation of IAP family proteins such as XIAP and survivin, and concomitant degradation of poly (ADP-ribose) polymerase. Moreover the activity of Akt was downregulated in EGL-treated cells, and the phosphatidylinositol-3 kinase/ Akt inhibitor LY294002 sensitized the cells to EGL-induced apoptosis. The results indicated that EGL induces the apoptosis of AGS cells through a signaling cascade of death receptor-mediated extrinsic, as well as mitochondria-mediated intrinsic, caspase pathways which are associated with inactivation of the Akt signal pathway.
doi_str_mv 10.1016/S2005-2901(10)60004-0
format article
fullrecord <record><control><sourceid>proquest_nrf_k</sourceid><recordid>TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_1077296</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S2005290110600040</els_id><sourcerecordid>733999081</sourcerecordid><originalsourceid>FETCH-LOGICAL-c511t-566c4f3923efb66fe8c0daecb635f7807272520b8bc51cdc87d29cf0f5c6bad23</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi1ERUvhJ4Bygx5Cx07tJCe0Wi3tSpUqlXI2ztgG08RebAfRf4-zu3DlNKOZZz70voS8ofCBAhWXnxkAr1kP9D2FCwEAVzU8I2cM-qbuKGfPl_yInJKXKf0AEIKy7gU5ZSCahlN2Rr5uvZ4xu-CrYKvVLuxySC5Vw1O1yd-VD2O1-Z2jwpwW4LpUtImTqsYZnZ6nyvnqZp6UL62Uo8NqrSI6HwqyNuOYXpETq8ZkXh_jOfnyafOwvqlv766369VtjZzSXHMh8Mo2PWuMHYSwpkPQyuAgGm7bDlrWMs5g6IbCo8au1axHC5ajGJRmzTm5OOz10cpHdDIot4_fgnyMcnX_sJUU2pb1orDvDuwuhp-zSVlOLmH5VnkT5iTbpun7HjpaSH4gMYaUorFyF92k4lPZJRcf5N4HuYi8lPY-SChzb48X5mEy-t_UX-EL8PEAmKLJL2eiTOiMR6NdNJilDu4_J_4AzcaXJA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733999081</pqid></control><display><type>article</type><title>Induction of Apoptosis by Ethanol Extracts of Ganoderma lucidum in Human Gastric Carcinoma Cells</title><source>BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS</source><creator>Jang, Kyung-Jun ; Han, Min-Ho ; Lee, Byung-Hoon ; Kim, Byung-Woo ; Kim, Cheol-Hong ; Yoon, Hyun-Min ; Choi, Yung-Hyun</creator><creatorcontrib>Jang, Kyung-Jun ; Han, Min-Ho ; Lee, Byung-Hoon ; Kim, Byung-Woo ; Kim, Cheol-Hong ; Yoon, Hyun-Min ; Choi, Yung-Hyun</creatorcontrib><description>Ganoderma lucidum, a well-known medicinal mushroom, is highly valued and commonly used in Oriental medicine. Although recent experimental data has revealed the proapoptotic potency of G. lucidum extracts, the underlying mechanisms of this apoptotic activity have not yet been studied in detail. In the present study, the effects of ethanol extracts of G. lucidum (EGL) on the growth of an AGS human gastric carcinoma cell line were investigated. We found that EGL treatment resulted in a dose and time-dependent significant decrease in the viability of AGS cells. This decreased viability was caused by apoptotic cell death, with observed chromatin condensation and an accumulation of apoptotic fraction. EGL treatment induced the expression of death receptor-related proteins such as death receptor 5 and tumor necrosis factor-related apoptosis-inducing ligand, which further triggered the activation of caspase-8 and the cleavage of Bid. In addition, the increase in apoptosis that was induced by EGL was correlated with activation of caspase-9 and -3, downregulation of IAP family proteins such as XIAP and survivin, and concomitant degradation of poly (ADP-ribose) polymerase. Moreover the activity of Akt was downregulated in EGL-treated cells, and the phosphatidylinositol-3 kinase/ Akt inhibitor LY294002 sensitized the cells to EGL-induced apoptosis. The results indicated that EGL induces the apoptosis of AGS cells through a signaling cascade of death receptor-mediated extrinsic, as well as mitochondria-mediated intrinsic, caspase pathways which are associated with inactivation of the Akt signal pathway.</description><identifier>ISSN: 2005-2901</identifier><identifier>EISSN: 2093-8152</identifier><identifier>DOI: 10.1016/S2005-2901(10)60004-0</identifier><identifier>PMID: 20633512</identifier><language>eng</language><publisher>Korea (South): 사단법인약침학회</publisher><subject>AGS ; Akt ; apoptosis ; Apoptosis - drug effects ; Carcinoma - drug therapy ; Carcinoma - enzymology ; Carcinoma - genetics ; Carcinoma - physiopathology ; caspase ; Caspases - metabolism ; Cell Line, Tumor ; Ganoderma lucidum ; Gene Expression Regulation, Neoplastic - drug effects ; Humans ; Plant Extracts - pharmacology ; Reishi - chemistry ; Signal Transduction - drug effects ; Stomach Neoplasms - drug therapy ; Stomach Neoplasms - enzymology ; Stomach Neoplasms - genetics ; Stomach Neoplasms - physiopathology ; 한의학</subject><ispartof>Journal of Acupuncture &amp; Meridian Studies, 2010, 3(1), 7, pp.24-31</ispartof><rights>2010 Korean Pharmacopuncture Institute</rights><rights>Copyright (c) 2010 Korean Pharmacopuncture Institute. Published by .. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-566c4f3923efb66fe8c0daecb635f7807272520b8bc51cdc87d29cf0f5c6bad23</citedby><cites>FETCH-LOGICAL-c511t-566c4f3923efb66fe8c0daecb635f7807272520b8bc51cdc87d29cf0f5c6bad23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20633512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001577309$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Jang, Kyung-Jun</creatorcontrib><creatorcontrib>Han, Min-Ho</creatorcontrib><creatorcontrib>Lee, Byung-Hoon</creatorcontrib><creatorcontrib>Kim, Byung-Woo</creatorcontrib><creatorcontrib>Kim, Cheol-Hong</creatorcontrib><creatorcontrib>Yoon, Hyun-Min</creatorcontrib><creatorcontrib>Choi, Yung-Hyun</creatorcontrib><title>Induction of Apoptosis by Ethanol Extracts of Ganoderma lucidum in Human Gastric Carcinoma Cells</title><title>Journal of acupuncture and meridian studies</title><addtitle>J Acupunct Meridian Stud</addtitle><description>Ganoderma lucidum, a well-known medicinal mushroom, is highly valued and commonly used in Oriental medicine. Although recent experimental data has revealed the proapoptotic potency of G. lucidum extracts, the underlying mechanisms of this apoptotic activity have not yet been studied in detail. In the present study, the effects of ethanol extracts of G. lucidum (EGL) on the growth of an AGS human gastric carcinoma cell line were investigated. We found that EGL treatment resulted in a dose and time-dependent significant decrease in the viability of AGS cells. This decreased viability was caused by apoptotic cell death, with observed chromatin condensation and an accumulation of apoptotic fraction. EGL treatment induced the expression of death receptor-related proteins such as death receptor 5 and tumor necrosis factor-related apoptosis-inducing ligand, which further triggered the activation of caspase-8 and the cleavage of Bid. In addition, the increase in apoptosis that was induced by EGL was correlated with activation of caspase-9 and -3, downregulation of IAP family proteins such as XIAP and survivin, and concomitant degradation of poly (ADP-ribose) polymerase. Moreover the activity of Akt was downregulated in EGL-treated cells, and the phosphatidylinositol-3 kinase/ Akt inhibitor LY294002 sensitized the cells to EGL-induced apoptosis. The results indicated that EGL induces the apoptosis of AGS cells through a signaling cascade of death receptor-mediated extrinsic, as well as mitochondria-mediated intrinsic, caspase pathways which are associated with inactivation of the Akt signal pathway.</description><subject>AGS</subject><subject>Akt</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Carcinoma - drug therapy</subject><subject>Carcinoma - enzymology</subject><subject>Carcinoma - genetics</subject><subject>Carcinoma - physiopathology</subject><subject>caspase</subject><subject>Caspases - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Ganoderma lucidum</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Humans</subject><subject>Plant Extracts - pharmacology</subject><subject>Reishi - chemistry</subject><subject>Signal Transduction - drug effects</subject><subject>Stomach Neoplasms - drug therapy</subject><subject>Stomach Neoplasms - enzymology</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - physiopathology</subject><subject>한의학</subject><issn>2005-2901</issn><issn>2093-8152</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhi1ERUvhJ4Bygx5Cx07tJCe0Wi3tSpUqlXI2ztgG08RebAfRf4-zu3DlNKOZZz70voS8ofCBAhWXnxkAr1kP9D2FCwEAVzU8I2cM-qbuKGfPl_yInJKXKf0AEIKy7gU5ZSCahlN2Rr5uvZ4xu-CrYKvVLuxySC5Vw1O1yd-VD2O1-Z2jwpwW4LpUtImTqsYZnZ6nyvnqZp6UL62Uo8NqrSI6HwqyNuOYXpETq8ZkXh_jOfnyafOwvqlv766369VtjZzSXHMh8Mo2PWuMHYSwpkPQyuAgGm7bDlrWMs5g6IbCo8au1axHC5ajGJRmzTm5OOz10cpHdDIot4_fgnyMcnX_sJUU2pb1orDvDuwuhp-zSVlOLmH5VnkT5iTbpun7HjpaSH4gMYaUorFyF92k4lPZJRcf5N4HuYi8lPY-SChzb48X5mEy-t_UX-EL8PEAmKLJL2eiTOiMR6NdNJilDu4_J_4AzcaXJA</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Jang, Kyung-Jun</creator><creator>Han, Min-Ho</creator><creator>Lee, Byung-Hoon</creator><creator>Kim, Byung-Woo</creator><creator>Kim, Cheol-Hong</creator><creator>Yoon, Hyun-Min</creator><creator>Choi, Yung-Hyun</creator><general>사단법인약침학회</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ACYCR</scope></search><sort><creationdate>20100301</creationdate><title>Induction of Apoptosis by Ethanol Extracts of Ganoderma lucidum in Human Gastric Carcinoma Cells</title><author>Jang, Kyung-Jun ; Han, Min-Ho ; Lee, Byung-Hoon ; Kim, Byung-Woo ; Kim, Cheol-Hong ; Yoon, Hyun-Min ; Choi, Yung-Hyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c511t-566c4f3923efb66fe8c0daecb635f7807272520b8bc51cdc87d29cf0f5c6bad23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>AGS</topic><topic>Akt</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Carcinoma - drug therapy</topic><topic>Carcinoma - enzymology</topic><topic>Carcinoma - genetics</topic><topic>Carcinoma - physiopathology</topic><topic>caspase</topic><topic>Caspases - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Ganoderma lucidum</topic><topic>Gene Expression Regulation, Neoplastic - drug effects</topic><topic>Humans</topic><topic>Plant Extracts - pharmacology</topic><topic>Reishi - chemistry</topic><topic>Signal Transduction - drug effects</topic><topic>Stomach Neoplasms - drug therapy</topic><topic>Stomach Neoplasms - enzymology</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - physiopathology</topic><topic>한의학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jang, Kyung-Jun</creatorcontrib><creatorcontrib>Han, Min-Ho</creatorcontrib><creatorcontrib>Lee, Byung-Hoon</creatorcontrib><creatorcontrib>Kim, Byung-Woo</creatorcontrib><creatorcontrib>Kim, Cheol-Hong</creatorcontrib><creatorcontrib>Yoon, Hyun-Min</creatorcontrib><creatorcontrib>Choi, Yung-Hyun</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Korean Citation Index</collection><jtitle>Journal of acupuncture and meridian studies</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jang, Kyung-Jun</au><au>Han, Min-Ho</au><au>Lee, Byung-Hoon</au><au>Kim, Byung-Woo</au><au>Kim, Cheol-Hong</au><au>Yoon, Hyun-Min</au><au>Choi, Yung-Hyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of Apoptosis by Ethanol Extracts of Ganoderma lucidum in Human Gastric Carcinoma Cells</atitle><jtitle>Journal of acupuncture and meridian studies</jtitle><addtitle>J Acupunct Meridian Stud</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>3</volume><issue>1</issue><spage>24</spage><epage>31</epage><pages>24-31</pages><issn>2005-2901</issn><eissn>2093-8152</eissn><abstract>Ganoderma lucidum, a well-known medicinal mushroom, is highly valued and commonly used in Oriental medicine. Although recent experimental data has revealed the proapoptotic potency of G. lucidum extracts, the underlying mechanisms of this apoptotic activity have not yet been studied in detail. In the present study, the effects of ethanol extracts of G. lucidum (EGL) on the growth of an AGS human gastric carcinoma cell line were investigated. We found that EGL treatment resulted in a dose and time-dependent significant decrease in the viability of AGS cells. This decreased viability was caused by apoptotic cell death, with observed chromatin condensation and an accumulation of apoptotic fraction. EGL treatment induced the expression of death receptor-related proteins such as death receptor 5 and tumor necrosis factor-related apoptosis-inducing ligand, which further triggered the activation of caspase-8 and the cleavage of Bid. In addition, the increase in apoptosis that was induced by EGL was correlated with activation of caspase-9 and -3, downregulation of IAP family proteins such as XIAP and survivin, and concomitant degradation of poly (ADP-ribose) polymerase. Moreover the activity of Akt was downregulated in EGL-treated cells, and the phosphatidylinositol-3 kinase/ Akt inhibitor LY294002 sensitized the cells to EGL-induced apoptosis. The results indicated that EGL induces the apoptosis of AGS cells through a signaling cascade of death receptor-mediated extrinsic, as well as mitochondria-mediated intrinsic, caspase pathways which are associated with inactivation of the Akt signal pathway.</abstract><cop>Korea (South)</cop><pub>사단법인약침학회</pub><pmid>20633512</pmid><doi>10.1016/S2005-2901(10)60004-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2005-2901
ispartof Journal of Acupuncture & Meridian Studies, 2010, 3(1), 7, pp.24-31
issn 2005-2901
2093-8152
language eng
recordid cdi_nrf_kci_oai_kci_go_kr_ARTI_1077296
source BACON - Elsevier - GLOBAL_SCIENCEDIRECT-OPENACCESS
subjects AGS
Akt
apoptosis
Apoptosis - drug effects
Carcinoma - drug therapy
Carcinoma - enzymology
Carcinoma - genetics
Carcinoma - physiopathology
caspase
Caspases - metabolism
Cell Line, Tumor
Ganoderma lucidum
Gene Expression Regulation, Neoplastic - drug effects
Humans
Plant Extracts - pharmacology
Reishi - chemistry
Signal Transduction - drug effects
Stomach Neoplasms - drug therapy
Stomach Neoplasms - enzymology
Stomach Neoplasms - genetics
Stomach Neoplasms - physiopathology
한의학
title Induction of Apoptosis by Ethanol Extracts of Ganoderma lucidum in Human Gastric Carcinoma Cells
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T18%3A04%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_nrf_k&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Induction%20of%20Apoptosis%20by%20Ethanol%20Extracts%20of%20Ganoderma%20lucidum%20in%20Human%20Gastric%20Carcinoma%20Cells&rft.jtitle=Journal%20of%20acupuncture%20and%20meridian%20studies&rft.au=Jang,%20Kyung-Jun&rft.date=2010-03-01&rft.volume=3&rft.issue=1&rft.spage=24&rft.epage=31&rft.pages=24-31&rft.issn=2005-2901&rft.eissn=2093-8152&rft_id=info:doi/10.1016/S2005-2901(10)60004-0&rft_dat=%3Cproquest_nrf_k%3E733999081%3C/proquest_nrf_k%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c511t-566c4f3923efb66fe8c0daecb635f7807272520b8bc51cdc87d29cf0f5c6bad23%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=733999081&rft_id=info:pmid/20633512&rfr_iscdi=true