Anti-septic activity of alpha-cubebenoate isolated from Schisandra chinensis

• Published in: BMB reports 2015, 48(6), , pp.336-341
• Main Authors: Kook, M., Sungkyunkwan University, Suwon, Republic of Korea, Lee, S.K., Sungkyunkwan University, Suwon, Republic of Korea, Kim, S.D., Sungkyunkwan University, Suwon, Republic of Korea, Lee, H.Y., Sungkyunkwan University, Suwon, Republic of Korea, Hwang, J.S., National Academy of Agricultural Science, RDA, Wanju, Republic of Korea, Choi, Y.W., Pusan National University, Miryang, Republic of Korea, Bae, Y.S., Sungkyunkwan University, Suwon, Republic of Korea
• Format: Article
• Language: English
• Subjects: alpha-Cubebenoate,Bactericidal activity,Inflammation,Sepsis; APOPTOSE; APOPTOSIS; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2015.48.6.202

Sepsis is a life-threatening, infectious, systemic inflammatory disease. In this study, we investigated the therapeutic effect of alpha-cubebenoate, a novel compound isolated from Schisandra chinensis against polymicrobial sepsis in a cecal ligation and puncture (CLP) experimental model. Administration of alpha-cubebenoate strongly enhanced survival in the CLP model. alpha-cubebenoate administration also markedly blocked CLP-induced lung inflammation and increased bactericidal activity by enhancing phagocytic activity and hydrogen peroxide generation in mouse bone marrow-derived macrophages and neutrophils. Expression of two important inflammatory cytokines, IL-1ƒÒ and IL-6, was strongly increased in the CLP model, and this was dramatically blocked by alpha-cubebenoate. Lymphocyte apoptosis and caspase-3 activation, which are associated with immune paralysis during sepsis, were markedly attenuated by alpha-cubebenoate. Taken together, our findings indicate that alpha-cubebenoate, a natural compound isolated from Schisandra chinensis, is a powerful potential anti-septic agent.