Anticoagulant activities of oleanolic acid via inhibition of tissue factor expressions

• Published in: BMB reports 2012, 45(7), , pp.390-395
• Main Authors: Lee, W.H., Kyungpook National University, Daegu, Republic of Korea, Yang, E.J., Kyungpook National University, Daegu, Republic of Korea, Ku, S.K., Daegu Haany University, Gyeongsan, Republic of Korea, Song, K.S., Kyungpook National University, Daegu, Republic of Korea, Bae, J.S., Kyungpook National University, Daegu, Republic of Korea
• Format: Article
• Language: English
• Subjects: aPTT; CELLS; CELLULE; CELULAS; Coagulation cascade; Oleanolic acid; Thrombin; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2012.45.7.065

Oleanolic acid (OA), a triterpenoid known for its anti-inflammatory and anti-cancer properties, is commonly present in several medicinal plants but its anticoagulant activities have not been studied. Here, the anticoagulant properties of OA were determined by monitoring activated partial thromboplastin time (aPTT), prothrombin time (PT), fibrin polymerization as well as cell-based thrombin and activated factor X (FXa) generation activities. Data showed OA prolonged aPTT and PT significantly and inhibited thrombin catalyzed fibrin polymerization. In addition, OA inhibited the activities of thrombin and FXa and inhibited the generation of thrombin or FXa in human endothelial cells. OA also inhibited TNF-α-induced tissue factor expression on human endothelial cells. In accordance with these anticoagulant activities, OA showed an anticoagulant effect in vivo. These results indicate that OA possesses antithrombotic activities and suggest that daily consumption of a herb containing OA may be preventing thrombosis in pathological states.