Proteome analysis of developing mice diastema region

Different from humans, who have a continuous dentition of teeth, mice have only three molars and one incisor separated by a toothless region called the diastema in the hemi mandibular arch. Although tooth buds form in the embryonic diastema, they regress and do not develop into teeth. In this study,...

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Published in:BMB reports 2012, 45(6), , pp.337-341
Main Authors: Chae, Y.M., Kyungpook National University, Daegu, Republic of Korea, Jin, Y.J., Kyungpook National University, Daegu, Republic of Korea, Kim, H.S., Kyungpook National University, Daegu, Republic of Korea, Gwon, G.J., Kyungpook National University, Daegu, Republic of Korea, Sohn, W.J., Kyungpook National University, Daegu, Republic of Korea, Kim, S.H., Kyungpook National University, Daegu, Republic of Korea, Kim, M.O., Kyungpook National University, Daegu, Republic of Korea, Lee, S.G., Kyungpook National University, Daegu, Republic of Korea, Suh, J.Y., Kyungpook National University, Daegu, Republic of Korea, Kim, J.Y., Kyungpook National University, Daegu, Republic of Korea
Format: Article
Language:English
Subjects:
DENT
Diastema
DIENTES
EMERIN
RAB7A
TEETH
Tooth development
화학
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Summary:Different from humans, who have a continuous dentition of teeth, mice have only three molars and one incisor separated by a toothless region called the diastema in the hemi mandibular arch. Although tooth buds form in the embryonic diastema, they regress and do not develop into teeth. In this study, we evaluated the proteins that modulate the diastema formation through comparative analysis with molar-forming tissue by liquid chromatography-tandem mass spectroscopy (LC-MS/MS) proteome analysis. From the comparative and semi-quantitative proteome analysis, we identified 147 up- and 173 down-regulated proteins in the diastema compared to the molar forming proteins. Based on this proteome analysis, we selected and evaluated two candidate proteins, EMERIN and RAB7A, as diastema tissue specific markers. This study provides the first list of proteins that were detected in the mouse embryonic diastema region, which will be useful to understand the mechanisms of tooth development.
ISSN:1976-6696
1976-670X
DOI:10.5483/BMBRep.2012.45.6.025