In-silico and In-vitro based studies of Streptomyces peucetius CYP107N3 for oleic acid epoxidation

Certain members of the cytochromes P450 superfamily metabolize polyunsaturated long-chain fatty acids to several classes of oxygenated metabolites. An approach based on in silico analysis predicted that Streptomyces peucetius CYP107N3 might be a fatty acid-metabolizing enzyme, showing high homology...

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Bibliographic Details
Published in:BMB reports 2012, 45(12), , pp.736-741
Main Authors: Bhattarai, Saurabh, SunMoon University, Asan, Republic of Korea, Niraula, Narayan Prasad, SunMoon University, Asan, Republic of Korea, Sohng, J.K., SunMoon University, Asan, Republic of Korea, Oh, T.J., SunMoon University, Asan, Republic of Korea
Format: Article
Language:English
Subjects:
CITOCROMO P450
CYTOCHROME P450
Epoxidation
Homology Modeling/ docking
In silico
Streptomyces peucetius
화학
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Summary:Certain members of the cytochromes P450 superfamily metabolize polyunsaturated long-chain fatty acids to several classes of oxygenated metabolites. An approach based on in silico analysis predicted that Streptomyces peucetius CYP107N3 might be a fatty acid-metabolizing enzyme, showing high homology with epoxidase enzymes. Homology modeling and docking studies of CYP107N3 showed that oleic acid can fit directly into the active site pocket of the double bond of oleic acid within optimum distance of 4.6 angstrom from the Fe. In order to confirm the epoxidation activity proposed by in silico analysis, a gene coding CYP107N3 was expressed in Escherichia coli. The purified CYP107N3 was shown to catalyze C∧9-C∧10 epoxidation of oleic acid in vitro to 9,10-epoxy stearic acid confirmed by ESI-MS, HPLC-MS and GC-MS spectral analysis.
ISSN:1976-6696
1976-670X
DOI:10.5483/BMBRep.2012.45.12.080