Enhanced biological effects of Phe140Asn, a novel human granulocyte colony-stimulating factor mutant, on HL60 cells

• Published in: BMB reports 2011, 44(10), , pp.686-691
• Main Authors: Chung, Hee-Kyoung, Kim, Sung-Woo, Byun, Sung-June, Ko, Eun-Mi, Chung, Hak-Jae, Woo, Jae-Seok, Yoo, Jae-Gyu, Lee, Hwi-Cheul, Yang, Byoung-Chul, Kwon, Moo-Sik, Park, Soo-Bong, Park, Jin-Ki, Kim, Kyung-Woon
• Format: Article
• Language: English
• Subjects: 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2011.44.10.686

Granulocyte colony-stimulating factor (G-CSF) is a cytokine secreted by stromal cells and plays a role in the differentiation of bone marrow stem cells and proliferation of neutrophils. Therefore, G-CSF is widely used to reduce the risk of serious infection in immunocompromised patients; however, its use in such patients is limited because of its non-persistent biological activity. We created an N-linked glycosylated form of this cytokine,hG-CSF (Phe140Asn), to assess its biological activity in the promyelocyte cell line HL60. Enhanced biological effects were identified by analyzing the JAK2/STAT3/survivin pathway in HL60 cells. In addition, mutant hG-CSF (Phe140Asn) was observed to have enhanced chemoattractant effects and improved differentiation efficiency in HL60 cells. These results suggest that the addition of N-linked glycosylation was successful in improving the biological activity of hG-CSF. Furthermore,the mutated product appears to be a feasible therapy for patients with neutropenia. [BMB reports 2011; 44(10): 686-691] KCI Citation Count: 4