Expression of Kip-related protein 4 gene (KRP4) in response to auxin and cytokinin during growth of Arabidopsis thaliana

• Published in: BMB reports 2010, 43(4), , pp.273-278
• Main Authors: Cho, H.J., Kangwon National University, Chuncheon, Republic of Korea, Kwon, H.K., Kangwon National University, Chuncheon, Republic of Korea, Wang, M.H., Kangwon National University, Chuncheon, Republic of Korea
• Format: Article
• Language: English
• Subjects: ARABIDOPSIS THALIANA; AUXINAS; AUXINE; AUXINS; CELL CYCLE; CICLO CELULAR; CITOQUININAS; CYCLE CELLULAIRE; CYTOKININE; CYTOKININS; Kip-related protein 4; 화학
• ISSN: 1976-6696; 1976-670X
• DOI: 10.5483/BMBRep.2010.43.4.273

The cell cycle is regulated by cyclin-dependent kinase (CDK)-cyclin complexes as well as other regulators. We isolated Kip-related protein 4 (KRP4) cDNA that encodes 289 amino acids including six conserved domains. To investigate the expression pattern of KRP4 as well as of other cell cycle-related genes associated with plant hormones, Arabidopsis seedlings were cultured on MS medium containing auxin or cytokinin. All seedlings treated with phytohormones displayed an increased proportion of cells in S phase. A higher proportion of cells in G2 phase was observed in seedlings treated with NAA. RT-PCR confirmed that the expression of KRP4 was decreased after treatment with phytohormones, and that CDKA and D-type cyclin transcription was increased. Additionally, mitotic cyclins were up-regulated by NAA treatment. These results suggest that KRP4 as well as other cell cycle-related genes might contribute to the control of plant growth in response to exogenous hormones.