Time-course analysis of DNA damage response-related genes after in vitro radiation in H460 and H1229 lung cancer cell lines

Radiation is the most useful treatment modality for cancer patients. It initiates a series of signal cascades such as DNA damage response (DDR) signaling for repairing damaged DNA, arresting the cell cycle, and inducing cell death. Until now, few genes have been found to be regulated by radiation, w...

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Published in:Experimental & molecular medicine 2011, 43(7), , pp.419-426
Main Authors: Kim, Kang Ho, Yoo, Hae Yong, Joo, Kyeung Min, Jung, Yong, Jin, Juyoun, Kim, Yonghyun, Yoon, Su Jin, Choi, Seung Ho, Seol, Ho Jun, Park, Woong-Yang, Nam, Do-Hyun
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - genetics
Biomedical and Life Sciences
Biomedicine
Cell Cycle Proteins - genetics
Cell Line, Tumor
Cell Survival - radiation effects
DNA Damage - radiation effects
DNA Repair Enzymes - genetics
DNA-Binding Proteins - genetics
Gene Expression Profiling
Gene Expression Regulation, Neoplastic - radiation effects
Humans
Lung cancer
Lung Neoplasms
Medical Biochemistry
Molecular Medicine
Original
Radiation Tolerance - genetics
Signal Transduction
Stem Cells
생화학
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Summary:Radiation is the most useful treatment modality for cancer patients. It initiates a series of signal cascades such as DNA damage response (DDR) signaling for repairing damaged DNA, arresting the cell cycle, and inducing cell death. Until now, few genes have been found to be regulated by radiation, which explains the molecular mechanisms of cellular responses to radiation. Although the transcriptional changes caused by radiation have been widely investigated, little is known about the direct evidence for the transcriptional control of DDR-related genes. Here, we examined the radiosensitivity of two non-small cell lung cancer cell lines (H460 and H1299), which have different p53 status. We monitored the time-dependent changes of 24 DDR-related gene expressions via microarray analysis. Based on the basal expression levels and temporal patterns, we further classified 24 DDR-related genes into four subgroups. Then, we also addressed the protein levels of several DDR-related genes such as TopBP1, Chk1 and Chk2, confirming the results of microarray analysis. Together, these results indicate that the expression patterns of DDR-related genes are associated with radiosensitivity and with the p53 statuses of H460 and H1299, which adds to the understanding of the complex biological responses to radiation.
ISSN:1226-3613
2092-6413
DOI:10.3858/emm.2011.43.7.046