Spatiotemporal regulation of fibroblast growth factor signal blocking for endoderm formation in Xenopus laevis

We have previously shown that the inhibition of fibroblast growth factor (FGF) signaling induced endodermal gene expression in the animal cap and caused the expansion of the endodermal mass in Xenopus embryos. However, we still do not know whether or not the alteration of FGF signaling controls embr...

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Bibliographic Details
Published in:Experimental & molecular medicine 2008, 40(5), , pp.550-558
Main Authors: Cha, Sang-wook, Lee, Jong-Woo, Hwang, Yoo-seok, Chae, Jeong-Pil, Park, Kwon Moo, Cho, Hee Jung, Kim, Dong Sun, Bae, Yong Chul, Park, Mae Ja
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Endoderm - drug effects
Endoderm - embryology
Endoderm - metabolism
Fibroblast Growth Factors - antagonists & inhibitors
Fibroblast Growth Factors - genetics
Fibroblast Growth Factors - physiology
Gene Expression Regulation, Developmental - drug effects
In Situ Hybridization
Medical Biochemistry
Molecular Medicine
Original
Pyrroles - administration & dosage
Pyrroles - pharmacology
Receptors, Fibroblast Growth Factor - genetics
Receptors, Fibroblast Growth Factor - physiology
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction - drug effects
Stem Cells
Xenopus laevis
Xenopus laevis - embryology
Xenopus laevis - genetics
Xenopus laevis - physiology
Xenopus Proteins - antagonists & inhibitors
Xenopus Proteins - genetics
Xenopus Proteins - physiology
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Summary:We have previously shown that the inhibition of fibroblast growth factor (FGF) signaling induced endodermal gene expression in the animal cap and caused the expansion of the endodermal mass in Xenopus embryos. However, we still do not know whether or not the alteration of FGF signaling controls embryonic cell fate, or when FGF signal blocking is required for endoderm formation in Xenopus . Here, we show that FGF signal blocking in embryonic cells causes their descendants to move into the endodermal region and to express endodermal genes. It is also interesting that blocking FGF signaling between fertilization and embryonic stage 10.5 promotes endoderm formation, but persistent FGF signaling blocking after stage 10.5 restricts endoderm formation and differentiation.
ISSN:1226-3613
2092-6413
DOI:10.3858/emm.2008.40.5.550