Cytotoxicity of temporary cements on bovine dental pulp-derived cells (bDPCs) using real-time cell analysis

• Published in: The journal of advanced prosthodontics 2015, 7(1), 26, pp.21-26
• Main Authors: Meral Arslan Malkoç, Necla Demİr, Abdulkadir Şengün, Şerife Buket Bozkurt, Sema Sezgin Hakki
• Format: Article
• Language: English
• Subjects: 치의학
• ISSN: 2005-7806; 2005-7814
• DOI: 10.4047/jap.2015.7.1.21

PURPOSE. To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs). MATERIALS AND METHODS. Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco’s modified eagle’s culture medium (DMEM) for 72 hours according to ISO 10993-5. A real-time cell analyzer was used to determine cell vitality. After seeding 200 of the cell suspensions into the wells of a 96-well plate, the bDPCs were cured with bioactive components released by the test materials and observed every 15 minutes for 98 hours. One-way ANOVA and Tukey-Kramer tests were used to analyze the results of the proliferation experiments. RESULTS. All tested temporary cements showed significant decreases in the bDPCs index. Rely X Temp E, GC Fuji Temp, and Rely X Temp NE were severely toxic at both time points (24 and 72 hours) (P.05); however, the cell viability was significantly reduced at 72 hours (P