Antilipidemic effects and gene expression profiling of the glycosaminoglycans from cricket in rats on a high fat diet

• Published in: Archives of pharmacal research 2016, 39(7), , pp.926-936
• Main Authors: Ahn, Mi Young, Hwang, Jae Sam, Kim, Min-Ji, Park, Kun-Koo
• Format: Article
• Language: English
• Subjects: Adipose Tissue - drug effects; Adipose Tissue - metabolism; Animals; Body Weight - drug effects; Body Weight - physiology; Diet, High-Fat - adverse effects; Fatty Acids - antagonists & inhibitors; Fatty Acids - metabolism; Gene Expression Profiling - methods; Glycosaminoglycans - isolation & purification; Glycosaminoglycans - pharmacology; Gryllidae; Hypolipidemic Agents - isolation & purification; Hypolipidemic Agents - pharmacology; Male; Medicine; Pharmacology/Toxicology; Pharmacy; Rats; Rats, Wistar; Research Article; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-016-0749-1

Glycosaminoglycan (GAG) from cricket ( Gryllus bimaculatus ) was studied as a potential health supplement. Antiatherosclerotic and antilipidemic effects of the GAG of G . bimaculatus (GbG, 5 or 10 mg/kg) were investigated in 15-week old Wistar rats treated with GbG for over a month. GbG produced a meaningful anti-edema effect with inhibition of C-reactive protein (CRP). Also, the weights of abdominal and epididymidal fat were also reduced in conjunction with a mild increase in body weight. Furthermore, the sero-biochemical parameters showed an antihyperlipidemic effect with decreased levels of phospholipid, AST, ALT, total cholesterol and glucose in a dose-dependent manner. In addition anticoagulant and antithrombotic effects were seen: platelet, thrombin time, prothrombin time and Factor I were increased with GbG treatment. Furthermore, the GbG treated rat group (at 10 mg/kg) compared to control, showed that 588 genes (test/control ratio >2.0) including lipocalin 2 (Lcn2) and alpha 2-macroglobulin (A2 m) were up-regulated, and 569 genes (test/control ratio >0.5) including stearoyl-coenzyme A desaturase 1 (Scd1) were down-regulated. Based on these results, GbG could potentially prevent or treat fatty liver or hyperlipidemia in rats on a high-fat diet.