Anti-inflammatory, antioxidant and anti-acetylcholinesterase activities of Bouvardia ternifolia: potential implications in Alzheimer’s disease

• Published in: Archives of pharmacal research 2015, 38(7), , pp.1369-1379
• Main Authors: García-Morales, Giovanni, Huerta-Reyes, Maira, González-Cortazar, Manasés, Zamilpa, Alejandro, Jiménez-Ferrer, Enrique, Silva-García, Raúl, Román-Ramos, Rubén, Aguilar-Rojas, Arturo
• Format: Article
• Language: English
• Subjects: acetylcholinesterase; Alzheimer disease; Alzheimer Disease - drug therapy; Alzheimer Disease - metabolism; Amyloid beta-Peptides - antagonists & inhibitors; anti-inflammatory activity; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - pharmacology; antioxidant activity; antioxidants; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bouvardia; Cell Survival - drug effects; chlorogenic acid; Cholinesterase Inhibitors - isolation & purification; Cholinesterase Inhibitors - pharmacology; Coumarins - isolation & purification; Coumarins - pharmacology; ethyl acetate; Flavonoids - pharmacology; Humans; inflammation; inhibitory concentration 50; Medicine; Neuroprotective Agents - isolation & purification; Neuroprotective Agents - pharmacology; neuroprotective effect; neurotoxicity; oleanolic acid; Peptide Fragments - antagonists & inhibitors; Pharmacology/Toxicology; Pharmacy; Phenols - isolation & purification; Phenols - pharmacology; Plant Components, Aerial - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; polyphenols; Research Article; Rubiaceae - chemistry; rutin; scopoletin; Triterpenes - isolation & purification; Triterpenes - pharmacology; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-015-0587-6

Bouvardia ternifolia has been used medicinally to treat inflammation. In the present study, we investigate the anti-Alzheimer’s potential effect of the hydroalcoholic extract of B. ternifolia through evaluation of anti-inflammatory and antioxidant activities, quantification of the percentage inhibition of acetylcholinesterase activity, protection effect against β-amyloid fibrillar-induce neurotoxicity, and the identification of the main constituents. Our results show that B. ternifolia extract and ethyl acetate fraction induced anti-inflammatory effects by reducing inflammation by >70 %, while antioxidant test revealed significant IC₅₀ values for flavonoid content fraction (30.67 ± 2.09 μg/ml) and ethyl acetate fraction (42.66 ± 0.93 μg/ml). The maximum inhibition of acetylcholinesterase was exhibited by scopoletin content fraction (38.43 ± 3.94 %), while ethyl acetate fraction exerted neuroprotective effect against β-amyloid peptide (83.97 ± 5.03 %). Phytochemical analysis, showed the presence of 3-O-quercetin glucopyranoside (415 mg/g), rutin (229.9 mg/g), ursolic and oleanolic acid (54 and 20.8 mg/g respectively), 3-O-quercetin rhamnopyranoside (12.8 mg/g), chlorogenic acid (9.5 mg/g), and scopoletin (1.38 mg/g). Our findings support the use of B. ternifolia since the extract induced significant neuroprotection against β-amyloid peptide, anti-inflammatory, antioxidant and anti-acetylcholinesterase effects that could be attributed to its contents of polyphenols, coumarins, and triterpenes, and encourage further studies for development of this extract as therapeutic agent in treatment of Alzheimer’s disease.