Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells

• Published in: Archives of pharmacal research 2013, 36(7), , pp.912-917
• Main Authors: Choi, Jiwon, Kang, Hyun Ju, Kim, Sung Zoo, Kwon, Tae Oh, Jeong, Seung-Il, Jang, Seon Il
• Format: Article
• Language: English
• Subjects: Antioxidants - isolation & purification; Antioxidants - pharmacology; Erythrocytes - drug effects; Erythrocytes - metabolism; Free Radicals - antagonists & inhibitors; Free Radicals - metabolism; Hemolysis - drug effects; Hemolysis - physiology; Humans; Kaempferols - isolation & purification; Kaempferols - pharmacology; Medicine; Morus; Oxidative Stress - drug effects; Oxidative Stress - physiology; Pharmacology/Toxicology; Pharmacy; Plant Leaves; Research Article; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-013-0090-x

We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2′-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.