In vitro evaluation of the antioxidant and cytotoxic activities of constituents of the mangrove Lumnitzera racemosa Willd

• Published in: Archives of pharmacal research 2015, 38(4), , pp.446-455
• Main Authors: Thao, Nguyen Phuong, Luyen, Bui Thi Thuy, Diep, Chau Ngoc, Tai, Bui Huu, Kim, Eun Ji, Kang, Hee Kyoung, Lee, Sang Hyun, Jang, Hae Dong, Cuong, Nguyen The, Thanh, Nguyen Van, Cuong, Nguyen Xuan, Nam, Nguyen Hoai, Minh, Chau Van, Kim, Young Ho
• Format: Article
• Language: English
• Subjects: Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Apoptosis - drug effects; Apoptosis - physiology; Combretaceae; Cytotoxins - chemistry; Cytotoxins - isolation & purification; Cytotoxins - pharmacology; Drug Evaluation, Preclinical - methods; HL-60 Cells; Humans; Medicine; Pharmacology/Toxicology; Pharmacy; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves; Research Article; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-014-0429-y

This study performed phytochemical and bioactive assessments of the mangrove Lumnitzera racemosa Willd. leaves. Bioassay-guided fractionation of the methanolic extracts led to the identification of thirty-six compounds ( 1 – 36 ), their structures were elucidated using detailed NMR spectroscopic and MS analysis. The extracts, fractions, and the isolated compounds were screened for potential antioxidant and cytotoxic activities. Antioxidant assays were performed using peroxyl radical-scavenging and reducing assays, whereas cytotoxicity was measured using MTT assays in HL-60 and Hel-299 cell lines. The methanolic extract, CH 2 Cl 2 and n -BuOH fractions (10.0 μg/mL) exhibited potent antioxidant activity, with Trolox equivalent (TE) values of 24.94 ± 0.59, 28.34 ± 0.20, and 27.09 ± 0.37 (μM), respectively. In addition, the isolated compounds exerted cytotoxic effects in a dose-dependent manner; compounds 1 and 14 exhibited the most potent cytotoxicity in HL-60 cells, with IC 50 values of 0.15 ± 0.29 and 0.60 ± 0.16 μM, respectively. To clarify the mechanism(s) behind these cytotoxic effects, we measured the time-dependent changes in apoptotic markers including the condensation and fragmentation of nuclear chromatin, and the downregulation of p-ERK1/2, p-AKT, and c-Myc levels.