Immune modulation of glycosaminoglycan derived from P. lewisi in TNF-a stimulated cells

• Published in: Archives of pharmacal research 2015, 38(11), , pp.1983-1991
• Main Authors: 안미영, 김순자, 김남정, 황재삼, 윤은영
• Format: Article
• Language: English
• Subjects: 약학
• ISSN: 0253-6269; 1976-3786

Poecilocoris lewisi (Korean name: ‘‘Kwangdaenolinjae’’)is a red-striped gold stink bug (insect) whichhas been used as a crude drug in traditional medicine ofEast Asia and Korea. In this study, ethanol extract andglycosaminoglycan from P. lewisi (Pl GAG), as an activesubstance among its components, were investigated fortheir potential anti-inflammatory actions. They were foundto be a potent inducer of nitric oxide (NO) production fromcalf pulmonary artery endothelial (CPAE) cells and astimulator of endothelial nitric oxide synthase in a dosedependentmanner. The anti-inflammatory activities werealso evaluated by determining the level of adhesionmolecules related to atherogenesis and pro-inflammatorycytokines, such as vascular cell adhesion molecule-1(VCAM-1), intercellular adhesion molecule-1 (ICAM-1),secretory phospholipase A2, and prostaglandin E2,stimulated by tumor necrosis factor (TNF)-a in humanumbilical vein endothelial cells (HUVEC). They alsoshowed inhibitory effects on vascular endothelial growthfactor (VEGF) production in HUVECs. Matrix metalloproteinases(MMP-2 and 9) were also inhibited by treatmentwith this extract or glycosaminoglycan. Furthermore,this GAG showed cytotoxicity against CT-26 colon cancercells whereas having no cytotoxicity in CHO normal cells. Monosaccharide (amino, acidic, neutral monosaccharides)composition of used GAG was characterized bytrimethylsilylated GC–MS analysis method. KCI Citation Count: 4