Isolation of a dihydrobenzofuran lignan, icariside E4, with an antinociceptive effect from Tabebuia roseo-alba (Ridley) Sandwith (Bignoniaceae) bark

• Published in: Archives of pharmacal research 2015, 38(6), , pp.950-956
• Main Authors: Ferreira-Júnior, Jesu C, Conserva, Lucia M, Lyra Lemos, Rosangela P, de Omena-Neta, Genilda C, Cavalcante-Neto, Araken, Barreto, Emiliano
• Format: Article
• Language: English
• Subjects: Acetic Acid; analgesic effect; Analgesics - isolation & purification; Analgesics - pharmacology; Animals; atropine; bark; Behavior, Animal - drug effects; Dose-Response Relationship, Drug; Formaldehyde; formalin; glibenclamide; Glycosides - isolation & purification; Glycosides - pharmacology; haloperidol; Hot Temperature; Injections, Intraperitoneal; KATP Channels - drug effects; lignans; Lignans - isolation & purification; Lignans - pharmacology; Male; Medicine; methanol; Mice; naloxone; nociception; pain; Pain - chemically induced; Pain - drug therapy; Pain Measurement; Pharmacology/Toxicology; Pharmacy; Plant Bark - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Research Article; Tabebuia; Tabebuia - chemistry; yohimbine; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-014-0468-4

The antinociceptive activity of icariside E₄, a dihydrobenzofuran-type lignan isolated from Tabebuia roseo-alba (Ridley) Sandwith (Bignoniaceae) bark, was evaluated in mice by using chemical and thermal models of nociception. Intraperitoneal (i.p.) administration of crude T. roseo-alba bark extract and its methanol fraction inhibited acetic acid-induced abdominal constriction in mice. Furthermore, i.p. administration of 0.1, 1, and 10 mg/kg of icariside E₄ reduced the number of writhes evoked by acetic acid injection by 46.9, 82.3, and 66.6 %, respectively. Icariside E₄ administration had no effect in the first phase of the formalin test, but it reduced nociceptive behavior in the second phase as indicated by a reduction in the licking time. Icariside E₄ did not modify thermal nociception in the hot-plate test model, suggesting that it had a peripheral antinociceptive action. The antinociceptive effect of icariside E₄ in the writhing test was reversed by pre-administration of glibenclamide, but not of naloxone, atropine, yohimbine, or haloperidol. Together, these results indicated that the antinociceptive activity of icariside E₄ from T. roseo-alba in models of chemical pain occurred through ATP-sensitive K⁺ channel-dependent mechanisms.