Oleanane-type triterpenoid saponins from the roots of Pulsatilla koreana and their apoptosis-inducing effects on HL-60 human promyelocytic leukemia cells

• Published in: Archives of pharmacal research 2013, 36(6), , pp.768-774
• Main Authors: Li, Wei, Ding, Yan, Sun, Ya Nan, Yan, Xi Tao, Yang, Seo Young, Choi, Chun Whan, Kim, Eun-Ji, Kang, Hee Kyoung, Kim, Young Ho
• Format: Article
• Language: English
• Subjects: Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis - drug effects; HL-60 Cells; Humans; Inhibitory Concentration 50; Leukemia, Promyelocytic, Acute - drug therapy; Leukemia, Promyelocytic, Acute - pathology; Medicine; Oleanolic Acid - chemistry; Oleanolic Acid - isolation & purification; Oleanolic Acid - pharmacology; Pharmacology/Toxicology; Pharmacy; Plant Extracts - pharmacology; Plant Roots; Pulsatilla - chemistry; Research Article; Saponins - chemistry; Saponins - isolation & purification; Saponins - pharmacology; Spectrum Analysis; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-013-0042-5

Twenty-four oleanane-type triterpenoid saponins were isolated from a methanol extract of the Pulsatilla koreana roots. Their structures were elucidated by comparing spectroscopic data to published values. Among them, compounds 8 – 12 and 20 – 24 significantly diminished the proliferation of HL-60 human promyelocytic leukemia cells with IC 50 values from 0.3 to 4.2 μM, whereas compounds 7 and 19 showed moderate cytotoxic activity. Furthermore, apoptotic characteristics such as chromatin condensation and increase in the population of sub-G1 hypodiploid cells were observed after the HL-60 cells were treated with these compounds.