Inhibitory effect of acteoside on melittin-induced catecholamine exocytosis through inhibition of Ca2+-dependent phospholipase A2 and extracellular Ca2+ influx in PC12 cells

To investigate the inhibitory effect of acteoside on the process of exocytosis induced by melittin, we measured Ca 2+ mobilization, arachidonic acid (AA) release and catecholamine exocytosis in PC12 chromaffin cells. Melittin significantly increased the intracellular Ca 2+ mobilization via receptor-...

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Bibliographic Details
Published in:Archives of pharmacal research 2015, 38(10), , pp.1913-1920
Main Authors: Song, Ho Sun, Ko, Myung Soo, Jo, Young Soo, Whang, Wan Kyunn, Sim, Sang Soo
Format: Article
Language:English
Subjects:
Medicine
Pharmacology/Toxicology
Pharmacy
Research Article
약학
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Summary:To investigate the inhibitory effect of acteoside on the process of exocytosis induced by melittin, we measured Ca 2+ mobilization, arachidonic acid (AA) release and catecholamine exocytosis in PC12 chromaffin cells. Melittin significantly increased the intracellular Ca 2+ mobilization via receptor-operated calcium channel but not the intracellular Ca 2+ release. It caused AA release via activation of Ca 2+ -dependent phospholipase A 2 (PLA 2 ) and catecholamine secretion in a dose-dependent manner. Acteoside dose-dependently inhibited the release of AA and intracellular Ca 2+ mobilization induced by melittin. Acteoside reduced the catecholamine release and raised the amount of intracellular chromogranin A which is co-released with catecholamine from melittin-stimulated PC12 cells. Taken together, our results suggest that acteoside could suppress the exocytosis via inhibition of Ca 2+ -dependent PLA 2 and extracellular Ca 2+ influx in PC12 cells stimulated by melittin.
ISSN:0253-6269
1976-3786
DOI:10.1007/s12272-015-0601-z