In vitro antioxidant and anti-inflammatory activities of Angelica decursiva

• Published in: Archives of pharmacal research 2012, 35(1), , pp.179-192
• Main Authors: Zhao, Dafang, Islam, Md. Nurul, Ahn, Bo Ra, Jung, Hyun Ah, Kim, Byung-Woo, Choi, Jae Sue
• Format: Article
• Language: English
• Subjects: Angelica - chemistry; Animals; Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Antioxidants - isolation & purification; Antioxidants - pharmacology; Cell Line; Cell Survival - drug effects; Dose-Response Relationship, Drug; Medicine; Mice; Pharmacology/Toxicology; Pharmacy; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Research Article; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-012-0120-0

Mounting evidences continue to support the involvement of oxidative/nitrosative stress and inflammation in the pathogenesis of many diseases. Plant constituents having antioxidant activities together with anti-inflammatory activities may provide better opportunities to develop anti-inflammatory agents. In view of this, we evaluated the antioxidant and antiinflammatory activities of methanolic extract of whole plants of Angelica decursiva , and its solvent soluble fractions via in vitro activities against lipopolysaccharide-induced nitric oxide (NO) production in RAW 264.7 cells, as well as in vitro scavenging activities against 1,1-diphenyl-2-picrylhydrazyl, 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid, NO, and peroxynitrite. Among the tested fractions, the ethyl acetate fraction was found as the most active antioxidant fraction together with significant anti-inflammatory effect. From the active ethyl acetate fraction, four coumarin derivatives consisting of nodakenin, nodakenetin, umbelliferone, and umbelliferone-6-carboxylic acid, along with a phenolic compound, vanillic acid, were isolated. Among them, umbelliferone 6-carboxylic acid and vanillic acid were isolated for the first time from this plant. In all antioxidant assays, vanillic acid showed the highest antioxidant potential followed by umbelliferone 6-carboxylic acid among the isolated compounds. In the anti-inflammatory assay, umbelliferone 6-carboxylic acid exhibited the highest inhibitory activity against lipopolysaccharide-induced NO production in RAW 264.7 cells with an IC 50 value of 72.98 μg/mL. Therefore, the present study reveals the potential antioxidant and antiinflammatory activities of whole plants of A. decursiva and its constituents, mainly umbelliferone 6-carboxylic acid, which could be used in the development of therapeutic and preventive agents for oxidative stress-related inflammatory diseases.