Biological activities of fructus arctii fermented with the basidiomycete Grifola frondosa

• Published in: Archives of pharmacal research 2010, 33(12), , pp.1943-1951
• Main Authors: Kim, Jin-Hwa, Bae, Jun-Tae, Song, Min-Hyeon, Lee, Geun-Su, Choe, Soo-Young, Pyo, Hyeong-Bae
• Format: Article
• Language: English
• Subjects: Antioxidants - chemistry; Antioxidants - metabolism; Antioxidants - pharmacology; Arctium - metabolism; beta-Glucosidase - metabolism; Cells, Cultured; Cosmetics - chemistry; Dermis - drug effects; Fermentation; Fibroblasts - drug effects; Grifola - chemistry; Grifola - genetics; Grifola - metabolism; Humans; Lipoxygenase - metabolism; Lipoxygenase Inhibitors - chemistry; Lipoxygenase Inhibitors - pharmacology; Matrix Metalloproteinase 1 - metabolism; Matrix Metalloproteinase Inhibitors; Medicine; Mycelium - metabolism; Pharmacology/Toxicology; Pharmacy; Plant Extracts - pharmacology; RNA, Messenger - genetics; RNA, Messenger - metabolism; Ultraviolet Rays; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-010-1209-y

Fructus arctii extract containing phenolic glycosides was cultured with Grifola frondosa mycelia to produce β-glucosidase and its biological activities were studied. This β-glucosidase converted the glycosides (arctiin and caffeic acid derivatives) into aglycones (arctigenin and caffeic acid). Fermented Fructus arctii extract (G-FAE) with G. frondosa had antioxidant and 5-lipoxygenase inhibitory activities. The photoprotective potential of G-FAE was tested in human dermal fibroblasts (HDF) exposed to ultra-violet A (UVA). It was revealed that G-FAE had an inhibitory effect on human interstitial collagenase (matrix metalloproteinase, MMP-1) expression in UVA-irradiated HDF. The treatment of UVA-irradiated HDF with G-FAE resulted in a dose-dependent decrease in the expression level of MMP-1 mRNA. G-FAE also showed notable stimulation of collagen biosynthetic activity for fibroblasts. These diverse functionalities suggest that G-FAE could be a promising cosmetic ingredient.