Adoptive immunotherapy of human gastric cancer with ex vivo expanded T cells

Surgical resection of gastric cancer has made significant progress, but majority of patients with advanced gastric cancer face relapse and die within five years. In this study, the antitumor activity of ex vivo expanded T cells against the human gastric cancer was evaluated in vitro and in vivo . Hu...

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Bibliographic Details
Published in:Archives of pharmacal research 2010, 33(11), , pp.1789-1795
Main Authors: Kim, Yeon Jin, Lim, Jaeseung, Kang, Jong Soon, Kim, Hwan Mook, Lee, Hong Kyung, Ryu, Hwa Sun, Kim, Jee Youn, Hong, Jin Tae, Kim, Youngsoo, Han, Sang-Bae
Format: Article
Language:English
Subjects:
Animals
Cell Line, Tumor
Cytokine-Induced Killer Cells - immunology
Cytokine-Induced Killer Cells - metabolism
Cytokine-Induced Killer Cells - transplantation
Cytotoxicity, Immunologic
Humans
Immunotherapy, Adoptive
Interferon-gamma - metabolism
Interleukins - immunology
Interleukins - metabolism
Medicine
Mice
Mice, Nude
Pharmacology/Toxicology
Pharmacy
Stomach Neoplasms - immunology
Stomach Neoplasms - metabolism
Stomach Neoplasms - therapy
T-Lymphocytes - immunology
Tumor Necrosis Factor-alpha - metabolism
Xenograft Model Antitumor Assays
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Summary:Surgical resection of gastric cancer has made significant progress, but majority of patients with advanced gastric cancer face relapse and die within five years. In this study, the antitumor activity of ex vivo expanded T cells against the human gastric cancer was evaluated in vitro and in vivo . Human peripheral blood mononuclear cells were cultured with IL-2-containing medium in anti-CD3 antibody-coated flasks for 5 days, followed by incubation in IL-2-containing medium for 9 days. The resulting populations were mostly CD3 + T cells (97%) and comprised 1% CD3 − CD56 + , 36% CD3 + CD56 + , 11% CD4 + , and 80% CD8 + . This heterogeneous cell population was also called cytokine-induced killer (CIK) cells. CIK cells strongly produced IFN-γ, moderately TNF-α, but not IL-2 and IL-4. At an effector-target cell ratio of 30:1, CIK cells destroyed 58% of MKN74 human gastric cancer cells, as measured by the 51 Cr-release assay. In addition, CIK cells at doses of 3 and 10 million cells per mouse inhibited 58% and 78% of MKN74 tumor growth in nude mouse xenograft assays, respectively. This study suggests that CIK cells may be used as an adoptive immunotherapy for gastric cancer patients.
ISSN:0253-6269
1976-3786
DOI:10.1007/s12272-010-1111-7