Metabolism of a New Neuroprotective Agent for Ischemia-Reperfusion Damage, KR-31543 in the Rats using Liquid Chromatography/Electrospray Mass Spectrometry

KR-31543,(2S,3R,4S)-6-amino-4-[N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amino]-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-2H-1-benzopyran is a new neuroprotetive agent for ischemia-reperfusion damage. The in vitro and in vivo metabolism of KR-31543 in rats has been studied by LC-e...

Full description

Saved in:
Bibliographic Details
Published in:Archives of pharmacal research 2002, 25(5), , pp.664-668
Main Authors: Kim, John, Ji, Hye-Young, Lee, Seung-Seok, Yoo, Sung-Eun, Kim, Sun-Ok, Lee, Dong-Ha, Lim, Hong, Lee, Hye-Suk
Format: Article
Language:Korean
Subjects:
약학
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites
container_end_page 668
container_issue 5
container_start_page 664
container_title Archives of pharmacal research
container_volume 25
creator Kim, John
Ji, Hye-Young
Lee, Seung-Seok
Yoo, Sung-Eun
Kim, Sun-Ok
Lee, Dong-Ha
Lim, Hong
Lee, Hye-Suk
description KR-31543,(2S,3R,4S)-6-amino-4-[N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amino]-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-2H-1-benzopyran is a new neuroprotetive agent for ischemia-reperfusion damage. The in vitro and in vivo metabolism of KR-31543 in rats has been studied by LC-electrospray mass spectrometry. Rat liver microsomal incubation of KR-31543 in the presence of NADPH resulted in the formation of a metabolite M1. M1 was identified as N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amine on the basis of LC-MS/MS analysis with the synthesized authentic standard. Rat CYP3A1 and 3A2 are the major CYP isozymes involved in the formation of M1.
format article
fullrecord <record><control><sourceid>nrf_kisti</sourceid><recordid>TN_cdi_nrf_kci_oai_kci_go_kr_ARTI_1138180</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>oai_kci_go_kr_ARTI_1138180</sourcerecordid><originalsourceid>FETCH-LOGICAL-k640-2fb9a7dbcb35a7193dae0eb49fede33221c8b61914397d8a65061ed2893c9ee73</originalsourceid><addsrcrecordid>eNotjctKw0AYhYMoWKvvMBsXgsG5JJPMMtRbbWshdh8myZ9kzGXizFTpq_i0htbF4YPDxzln3oyIiPssivm5N8M0ZD6nXFx6V9Z-Ysx4GIYz73cDTua6U7ZHukISvcPPlL3Ro9EOCqe-ASU1DA5V2qClLRrolfRTGMFUe6v0gB5lL2u4R6vUZyQMGFIDcg2gVDqLJmWo0Vp97VWJFo3RvXS6NnJsDg9P3XRgtB2NPKCNtBZ9jMemB2cO195FJTsLN_-ce7vnp93i1V9vX5aLZO23PMA-rXIhozIvchbKiAhWSsCQB6KCEhijlBRxzokgARNRGUseYk6gpLFghQCI2Ny7O80OpsraQmVaqiNrnbUmS9LdMiOExSTGk3t7cltlncqG0nbZW7LaUowpIYJSyhmmAfsDobxzUA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Metabolism of a New Neuroprotective Agent for Ischemia-Reperfusion Damage, KR-31543 in the Rats using Liquid Chromatography/Electrospray Mass Spectrometry</title><source>Springer Nature</source><creator>Kim, John ; Ji, Hye-Young ; Lee, Seung-Seok ; Yoo, Sung-Eun ; Kim, Sun-Ok ; Lee, Dong-Ha ; Lim, Hong ; Lee, Hye-Suk</creator><creatorcontrib>Kim, John ; Ji, Hye-Young ; Lee, Seung-Seok ; Yoo, Sung-Eun ; Kim, Sun-Ok ; Lee, Dong-Ha ; Lim, Hong ; Lee, Hye-Suk</creatorcontrib><description>KR-31543,(2S,3R,4S)-6-amino-4-[N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amino]-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-2H-1-benzopyran is a new neuroprotetive agent for ischemia-reperfusion damage. The in vitro and in vivo metabolism of KR-31543 in rats has been studied by LC-electrospray mass spectrometry. Rat liver microsomal incubation of KR-31543 in the presence of NADPH resulted in the formation of a metabolite M1. M1 was identified as N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amine on the basis of LC-MS/MS analysis with the synthesized authentic standard. Rat CYP3A1 and 3A2 are the major CYP isozymes involved in the formation of M1.</description><identifier>ISSN: 0253-6269</identifier><identifier>EISSN: 1976-3786</identifier><language>kor</language><publisher>대한약학회</publisher><subject>약학</subject><ispartof>Archives of Pharmacal Research, 2002, 25(5), , pp.664-668</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885</link.rule.ids><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001192539$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, John</creatorcontrib><creatorcontrib>Ji, Hye-Young</creatorcontrib><creatorcontrib>Lee, Seung-Seok</creatorcontrib><creatorcontrib>Yoo, Sung-Eun</creatorcontrib><creatorcontrib>Kim, Sun-Ok</creatorcontrib><creatorcontrib>Lee, Dong-Ha</creatorcontrib><creatorcontrib>Lim, Hong</creatorcontrib><creatorcontrib>Lee, Hye-Suk</creatorcontrib><title>Metabolism of a New Neuroprotective Agent for Ischemia-Reperfusion Damage, KR-31543 in the Rats using Liquid Chromatography/Electrospray Mass Spectrometry</title><title>Archives of pharmacal research</title><addtitle>Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea</addtitle><description>KR-31543,(2S,3R,4S)-6-amino-4-[N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amino]-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-2H-1-benzopyran is a new neuroprotetive agent for ischemia-reperfusion damage. The in vitro and in vivo metabolism of KR-31543 in rats has been studied by LC-electrospray mass spectrometry. Rat liver microsomal incubation of KR-31543 in the presence of NADPH resulted in the formation of a metabolite M1. M1 was identified as N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amine on the basis of LC-MS/MS analysis with the synthesized authentic standard. Rat CYP3A1 and 3A2 are the major CYP isozymes involved in the formation of M1.</description><subject>약학</subject><issn>0253-6269</issn><issn>1976-3786</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNotjctKw0AYhYMoWKvvMBsXgsG5JJPMMtRbbWshdh8myZ9kzGXizFTpq_i0htbF4YPDxzln3oyIiPssivm5N8M0ZD6nXFx6V9Z-Ysx4GIYz73cDTua6U7ZHukISvcPPlL3Ro9EOCqe-ASU1DA5V2qClLRrolfRTGMFUe6v0gB5lL2u4R6vUZyQMGFIDcg2gVDqLJmWo0Vp97VWJFo3RvXS6NnJsDg9P3XRgtB2NPKCNtBZ9jMemB2cO195FJTsLN_-ce7vnp93i1V9vX5aLZO23PMA-rXIhozIvchbKiAhWSsCQB6KCEhijlBRxzokgARNRGUseYk6gpLFghQCI2Ny7O80OpsraQmVaqiNrnbUmS9LdMiOExSTGk3t7cltlncqG0nbZW7LaUowpIYJSyhmmAfsDobxzUA</recordid><startdate>200210</startdate><enddate>200210</enddate><creator>Kim, John</creator><creator>Ji, Hye-Young</creator><creator>Lee, Seung-Seok</creator><creator>Yoo, Sung-Eun</creator><creator>Kim, Sun-Ok</creator><creator>Lee, Dong-Ha</creator><creator>Lim, Hong</creator><creator>Lee, Hye-Suk</creator><general>대한약학회</general><scope>JDI</scope><scope>ACYCR</scope></search><sort><creationdate>200210</creationdate><title>Metabolism of a New Neuroprotective Agent for Ischemia-Reperfusion Damage, KR-31543 in the Rats using Liquid Chromatography/Electrospray Mass Spectrometry</title><author>Kim, John ; Ji, Hye-Young ; Lee, Seung-Seok ; Yoo, Sung-Eun ; Kim, Sun-Ok ; Lee, Dong-Ha ; Lim, Hong ; Lee, Hye-Suk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-k640-2fb9a7dbcb35a7193dae0eb49fede33221c8b61914397d8a65061ed2893c9ee73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>kor</language><creationdate>2002</creationdate><topic>약학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, John</creatorcontrib><creatorcontrib>Ji, Hye-Young</creatorcontrib><creatorcontrib>Lee, Seung-Seok</creatorcontrib><creatorcontrib>Yoo, Sung-Eun</creatorcontrib><creatorcontrib>Kim, Sun-Ok</creatorcontrib><creatorcontrib>Lee, Dong-Ha</creatorcontrib><creatorcontrib>Lim, Hong</creatorcontrib><creatorcontrib>Lee, Hye-Suk</creatorcontrib><collection>KoreaScience</collection><collection>Korean Citation Index</collection><jtitle>Archives of pharmacal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, John</au><au>Ji, Hye-Young</au><au>Lee, Seung-Seok</au><au>Yoo, Sung-Eun</au><au>Kim, Sun-Ok</au><au>Lee, Dong-Ha</au><au>Lim, Hong</au><au>Lee, Hye-Suk</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolism of a New Neuroprotective Agent for Ischemia-Reperfusion Damage, KR-31543 in the Rats using Liquid Chromatography/Electrospray Mass Spectrometry</atitle><jtitle>Archives of pharmacal research</jtitle><addtitle>Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea</addtitle><date>2002-10</date><risdate>2002</risdate><volume>25</volume><issue>5</issue><spage>664</spage><epage>668</epage><pages>664-668</pages><issn>0253-6269</issn><eissn>1976-3786</eissn><abstract>KR-31543,(2S,3R,4S)-6-amino-4-[N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amino]-3,4-dihydro-2-dimethoxymethyl-3-hydroxy-2-methyl-2H-1-benzopyran is a new neuroprotetive agent for ischemia-reperfusion damage. The in vitro and in vivo metabolism of KR-31543 in rats has been studied by LC-electrospray mass spectrometry. Rat liver microsomal incubation of KR-31543 in the presence of NADPH resulted in the formation of a metabolite M1. M1 was identified as N-(4-chlorophenyl)-N-(2-methyl-2H-tetrazol-5-ylmethyl)amine on the basis of LC-MS/MS analysis with the synthesized authentic standard. Rat CYP3A1 and 3A2 are the major CYP isozymes involved in the formation of M1.</abstract><pub>대한약학회</pub><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0253-6269
ispartof Archives of Pharmacal Research, 2002, 25(5), , pp.664-668
issn 0253-6269
1976-3786
language kor
recordid cdi_nrf_kci_oai_kci_go_kr_ARTI_1138180
source Springer Nature
subjects 약학
title Metabolism of a New Neuroprotective Agent for Ischemia-Reperfusion Damage, KR-31543 in the Rats using Liquid Chromatography/Electrospray Mass Spectrometry
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-08T03%3A38%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-nrf_kisti&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Metabolism%20of%20a%20New%20Neuroprotective%20Agent%20for%20Ischemia-Reperfusion%20Damage,%20KR-31543%20in%20the%20Rats%20using%20Liquid%20Chromatography/Electrospray%20Mass%20Spectrometry&rft.jtitle=Archives%20of%20pharmacal%20research&rft.au=Kim,%20John&rft.date=2002-10&rft.volume=25&rft.issue=5&rft.spage=664&rft.epage=668&rft.pages=664-668&rft.issn=0253-6269&rft.eissn=1976-3786&rft_id=info:doi/&rft_dat=%3Cnrf_kisti%3Eoai_kci_go_kr_ARTI_1138180%3C/nrf_kisti%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-k640-2fb9a7dbcb35a7193dae0eb49fede33221c8b61914397d8a65061ed2893c9ee73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true