Tectorigenin inhibits IFN-γ/LPS-induced inflammatory responses in murine macrophage RAW 264.7 cells

• Published in: Archives of pharmacal research 2008, 31(11), , pp.1447-1456
• Main Authors: Pan, Cheol-Ho, Kim, Eun Sun, Jung, Sang Hoon, Nho, Chu Won, Lee, Jae Kwon
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Blotting, Western; Cell Line; Cyclooxygenase 2 - metabolism; Dinoprostone - metabolism; Enzyme-Linked Immunosorbent Assay; Genistein - pharmacology; I-kappa B Kinase - metabolism; Indicators and Reagents; Interferon-gamma - antagonists & inhibitors; Interleukin-1beta - metabolism; Isoflavones - pharmacology; Lipopolysaccharides - antagonists & inhibitors; Macrophages - drug effects; Macrophages - pathology; Medicine; Mice; Nitric Oxide - metabolism; Nitric Oxide Synthase Type II - metabolism; Pharmacology/Toxicology; Pharmacy; Phytoestrogens - pharmacology; Research Article; Reverse Transcriptase Polymerase Chain Reaction; Tumor Necrosis Factor-alpha - metabolism; 약학
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-001-2129-7

Tectorigenin (Tg) and tectoridin (Td) are the major compounds isolated from the rhizomes of iridaceous plant Belamcanda chinensis which is well known as a chinese traditional medicine for the treatment of inflammatory diseases. In this study we investigated whether tectorigenin and tectoridin can be applied to the suppression of interferon-γ and lipopolysaccharide (IFN-γ/LPS)-induced inflammatory responses in macrophages. Anti-inflammatory activities of tectorigenin and tectoridin were compared with genistein (Ge), well known isoflavonoid as a phytoestrogen and regarded as an emerging anti-inflammatory agent. Both compounds showed low cytotoxic effect. In Raw 264.7 cells activated with IFN-γ/LPS, pre-treated tectorigenin was found to inhibit the expression of inducible nitric oxide synthase (iNOS), the production of nitric oxide (NO) and the secretion of interleukin (IL)-1β dose-dependently. Tectorigenin also decreased the expression of cyclooxigenase (COX)-2 and the production of prostaglandin E 2 (PGE 2 ) in dose-dependent manner. These inhibitory effects of tectorigenin were found to be caused by the blocking of nuclear factor kappa-B (NF-κB) activation. Compared with genistein and tectoridin, tectorigenin showed significant inhibitory effect for almost anti-inflammatory tests in this study. All these results clearly demonstrated that tectorigenin appears to have the potential to prevent inflammation.